biostudies-literatureUnknown43(6)McDonald SCFrogeye leaf spot is a yield-reducing disease of soybean caused by the pathogen <i>Cercospora sojina</i>. <i>Rcs3</i> has provided durable resistance to all known races of <i>C. sojina</i> since its discovery in the cultivar Davis during the 1980s. Using a recombinant inbred line population derived from a cross between Davis and the susceptible cultivar Forrest, <i>Rcs3</i> was fine-mapped to a 1.15 Mb interval on chromosome 16. This single locus was confirmed by tracing <i>Rcs3</i> in resistant and susceptible progeny derived from Davis, as well as three near-isogenic lines. Haplotype analysis in the ancestors of Davis indicated that Davis has the same haplotype at the <i>Rcs3</i> locus as susceptible cultivars in its paternal lineage. On the basis of these results, it is hypothesized thaMolecular breeding : new strategies in plant improvement49https://www.ebi.ac.uk/biostudies/studies/S-EPMC10248600biostudies-literaturePinpointing <i>Rcs3</i> for frogeye leaf spot resistance and tracing its origin in soybean breeding.PMC10248600Li ZBuck JWMcDonald SCfalsePinpointing <i>Rcs3</i> for frogeye leaf spot resistance and tracing its origin in soybean breeding.Frogeye leaf spot is a yield-reducing disease of soybean caused by the pathogen <i>Cercospora sojina</i>. <i>Rcs3</i> has provided durable resistance to all known races of <i>C. sojina</i> since its discovery in the cultivar Davis during the 1980s. Using a recombinant inbred line population derived from a cross between Davis and the susceptible cultivar Forrest, <i>Rcs3</i> was fine-mapped to a 1.15 Mb interval on chromosome 16. This single locus was confirmed by tracing <i>Rcs3</i> in resistant and susceptible progeny derived from Davis, as well as three near-isogenic lines. Haplotype analysis in the ancestors of Davis indicated that Davis has the same haplotype at the <i>Rcs3</i> locus as susceptible cultivars in its paternal lineage. On the basis of these results, it is hypothesized tha2023-01-01T00:00:00Z2023 Jun2026-05-28T13:01:18.795Z2026-04-08T02:40:43.976ZS-EPMC102486003731322510.1007/s11032-023-01397-x