biostudies-literatureUnknown11Budama-Kilinc Y<i>Onopordum acanthium</i> is a medicinal plant with many important properties, such as antibacterial, anticancer, and anti-hypotensive properties. Although various studies reported the biological activities of <i>O. acanthium</i>, there is no study on its nano-phyto-drug formulation. The aim of this study is to develop a candidate nano-drug based on phytotherapeutic constituents and evaluate its efficiency <i>in vitro</i> and <i>in silico</i>. In this context, poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) of <i>O. acanthium</i> extract (OAE) were synthesized and characterized. It was determined that the average particle size of OAE-PLGA-NPs was 214.9 ± 6.77 nm, and the zeta potential was -8.03 ± 0.85 mV, and PdI value was 0.064 ± 0.013. The encapsulation efficiency of OAE-PLGAPeerJe15523https://www.ebi.ac.uk/biostudies/studies/S-EPMC10257901biostudies-literature<i>In vitro</i> and <i>in silico</i> evaluation of the design of nano-phyto-drug candidate for oral use against <i>Staphylococcus aureus</i>.PMC10257901Budama-Kilinc YCetin Aluc CGok BKecel-Gunduz Sfalse<i>In vitro</i> and <i>in silico</i> evaluation of the design of nano-phyto-drug candidate for oral use against <i>Staphylococcus aureus</i>.<i>Onopordum acanthium</i> is a medicinal plant with many important properties, such as antibacterial, anticancer, and anti-hypotensive properties. Although various studies reported the biological activities of <i>O. acanthium</i>, there is no study on its nano-phyto-drug formulation. The aim of this study is to develop a candidate nano-drug based on phytotherapeutic constituents and evaluate its efficiency <i>in vitro</i> and <i>in silico</i>. In this context, poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) of <i>O. acanthium</i> extract (OAE) were synthesized and characterized. It was determined that the average particle size of OAE-PLGA-NPs was 214.9 ± 6.77 nm, and the zeta potential was -8.03 ± 0.85 mV, and PdI value was 0.064 ± 0.013. The encapsulation efficiency of OAE-PLGA2023-01-01T00:00:00Z20232026-05-28T20:12:55.026Z2024-11-19T23:17:25.162ZS-EPMC102579013730937110.7717/peerj.15523