biostudies-literatureUnknown15(10)Lei YB-cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) is overexpressed in various cancer types. We found that <i>Bmi-1</i> mRNA levels were elevated in nasopharyngeal carcinoma (NPC) cell lines. In immunohistochemical analyses, high Bmi-1 levels were observed in not only 5 of 38 non-cancerous nasopharyngeal squamous epithelial biopsies, but also in 66 of 98 NPC specimens (67.3%). High Bmi-1 levels were detected more frequently in T3-T4, N2-N3 and stage III-IV NPC biopsies than in T1-T2, N0-N1 and stage I-II NPC samples, indicating that Bmi-1 is upregulated in advanced NPC. In 5-8F and SUNE1 NPC cells, stable depletion of Bmi-1 using lentiviral RNA interference greatly suppressed cell proliferation, induced G1-phase cell cycle arrest, reduced cell stemness and suppressed Aging4391-4410https://www.ebi.ac.uk/biostudies/studies/S-EPMC10258013biostudies-literatureHairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating <i>Bmi-1</i>.PMC10258013Zhou ZHHe DHXia JWXiao SJLin XLChen MLWang SCZhou YLi XFDai GQCong JGLi YCZhang XKLi QWLei YXie HTHuang SHWu ABXiao DYang SJia JSShen HFLin TYfalseHairy gene homolog increases nasopharyngeal carcinoma cell stemness by upregulating <i>Bmi-1</i>.B-cell-specific Moloney murine leukemia virus integration site 1 (Bmi-1) is overexpressed in various cancer types. We found that <i>Bmi-1</i> mRNA levels were elevated in nasopharyngeal carcinoma (NPC) cell lines. In immunohistochemical analyses, high Bmi-1 levels were observed in not only 5 of 38 non-cancerous nasopharyngeal squamous epithelial biopsies, but also in 66 of 98 NPC specimens (67.3%). High Bmi-1 levels were detected more frequently in T3-T4, N2-N3 and stage III-IV NPC biopsies than in T1-T2, N0-N1 and stage I-II NPC samples, indicating that Bmi-1 is upregulated in advanced NPC. In 5-8F and SUNE1 NPC cells, stable depletion of Bmi-1 using lentiviral RNA interference greatly suppressed cell proliferation, induced G1-phase cell cycle arrest, reduced cell stemness and suppressed 2023-01-01T00:00:00Z2023 May2025-06-01T00:25:57.724Z2025-06-01T00:25:57.724ZS-EPMC102580133721944910.18632/aging.204742