biostudies-literatureWang JNatural Science Foundation of Henan ProvinceNational Natural Science Foundation of ChinaHainan Provincial Natural Science Foundation of China and Program for Science &amp; Technology Innovation Talents in Higher Education of Henan Province (23HASTIT050) Higher Education of Henan province1844-1862https://www.ebi.ac.uk/biostudies/studies/S-EPMC10262811biostudies-literatureUnknown19(6)<i>L. monocytogenes</i> is a widely used infection model for the research on pathogenesis and host defense against gram-positive intracellular bacteria. Emerging evidence indicates that posttranslational modifications play a critical role in the regulation of macroautophagy/autophagy. However, little is known about the posttranslational modifications of ATG7, the essential protein in the autophagy process. In this study, we demonstrated that the RING-type E3 ligase TRIM7/RNF90 positively regulated autophagosome accumulation by promoting the ubiquitination of ATG7 at K413, thereby affecting <i>L. monocytogenes</i> infection. TRIM7 expression was induced by a variety range of conditions, including starvation, rapamycin stimulation, and <i>L. monocytogenes</i> infection. TRIM7 deficiency in mAutophagyTRIM7/RNF90 promotes autophagy via regulation of ATG7 ubiquitination during <i>L. monocytogenes</i> infection.PMC10262811U1704183and 3217087121230041006531970847U200410332070949Ma SGoren IQin XPei JYang BWang HWang JZhang QLiu YHuang YXing HWang YSong ZfalseTRIM7/RNF90 promotes autophagy via regulation of ATG7 ubiquitination during <i>L. monocytogenes</i> infection.<i>L. monocytogenes</i> is a widely used infection model for the research on pathogenesis and host defense against gram-positive intracellular bacteria. Emerging evidence indicates that posttranslational modifications play a critical role in the regulation of macroautophagy/autophagy. However, little is known about the posttranslational modifications of ATG7, the essential protein in the autophagy process. In this study, we demonstrated that the RING-type E3 ligase TRIM7/RNF90 positively regulated autophagosome accumulation by promoting the ubiquitination of ATG7 at K413, thereby affecting <i>L. monocytogenes</i> infection. TRIM7 expression was induced by a variety range of conditions, including starvation, rapamycin stimulation, and <i>L. monocytogenes</i> infection. TRIM7 deficiency in m2023-01-01T00:00:00Z2023 Jun2026-06-04T08:05:37.17Z2025-04-21T15:12:21.833ZS-EPMC102628113657615010.1080/15548627.2022.2162706