biostudies-literatureKato MNIDDK NIH HHSNational Institute of Diabetes and Digestive and Kidney DiseasesNHLBI NIH HHSNCI NIH HHS1204124https://www.ebi.ac.uk/biostudies/studies/S-EPMC10266347biostudies-literatureUnknown10<b>Background:</b> MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play key roles in diabetic kidney disease (DKD). The miR-379 megacluster of miRNAs and its host transcript lnc-megacluster (lncMGC) are regulated by transforming growth factor-β (TGF-β), increased in the glomeruli of diabetic mice, and promote features of early DKD. However, biochemical functions of lncMGC are unknown. Here, we identified lncMGC-interacting proteins by <i>in vitro</i>-transcribed lncMGC RNA pull down followed by mass spectrometry. We also created lncMGC-knockout (KO) mice by CRISPR-Cas9 editing and used primary mouse mesangial cells (MMCs) from the KO mice to examine the effects of lncMGC on the gene expression related to DKD, changes in promoter histone modifications, and chromatin remodeling. <b>MetFrontiers in molecular biosciencesLong non-coding RNA lncMGC mediates the expression of TGF-β-induced genes in renal cells via nucleosome remodelers.PMC10266347R01 DK065073R01 HL106089R01DK081705 R01DK065073 R01HL106089P30 CA033572R01 DK081705Kalkum MAbdollahi MDas STsark WMoore RWang JLanting LWang MChen ZKato MChen WLi ATunduguru RNatarajan RWu XfalseLong non-coding RNA lncMGC mediates the expression of TGF-β-induced genes in renal cells via nucleosome remodelers.<b>Background:</b> MicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) play key roles in diabetic kidney disease (DKD). The miR-379 megacluster of miRNAs and its host transcript lnc-megacluster (lncMGC) are regulated by transforming growth factor-β (TGF-β), increased in the glomeruli of diabetic mice, and promote features of early DKD. However, biochemical functions of lncMGC are unknown. Here, we identified lncMGC-interacting proteins by <i>in vitro</i>-transcribed lncMGC RNA pull down followed by mass spectrometry. We also created lncMGC-knockout (KO) mice by CRISPR-Cas9 editing and used primary mouse mesangial cells (MMCs) from the KO mice to examine the effects of lncMGC on the gene expression related to DKD, changes in promoter histone modifications, and chromatin remodeling. <b>Met2023-01-01T00:00:00Z20232026-05-29T19:26:01.376Z2024-11-12T22:04:24.195ZS-EPMC102663473732547010.3389/fmolb.2023.1204124