{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Li J"],"funding":["NIAID NIH HHS","HHS | NIH | National Institute of Allergy and Infectious Diseases"],"pagination":["e0005323"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10269042"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["91(6)"],"pubmed_abstract":["Clostridium perfringens type F strains cause food poisoning (FP) when they sporulate and produce C. perfringens enterotoxin (CPE) in the intestines. Most type F FP strains carry a chromosomal <i>cpe</i> gene (c-<i>cpe</i> strains). C. perfringens produces up to three different sialidases, named NanH, NanI, and NanJ, but some c-<i>cpe</i> FP strains carry only <i>nanJ</i> and <i>nanH</i> genes. This study surveyed a collection of such strains and showed that they produce sialidase activity when cultured in Todd-Hewitt broth (TH) (vegetative cultures) or modified Duncan-Strong (MDS) medium (sporulating cultures). Sialidase null mutants were constructed in 01E809, a type F c-<i>cpe</i> FP strain carrying the <i>nanJ</i> and <i>nanH</i> genes. Characterization of those mutants identified NanJ "],"journal":["Infection and immunity"],"pubmed_title":["NanJ Is the Major Sialidase for Clostridium perfringens Type F Food Poisoning Strain 01E809."],"pmcid":["PMC10269042"],"funding_grant_id":["R01AI019844","R21 AI146492","R21AI146492","R01 AI019844"],"pubmed_authors":["Pradhan A","Li J","McClane BA"],"additional_accession":[]},"is_claimable":false,"name":"NanJ Is the Major Sialidase for Clostridium perfringens Type F Food Poisoning Strain 01E809.","description":"Clostridium perfringens type F strains cause food poisoning (FP) when they sporulate and produce C. perfringens enterotoxin (CPE) in the intestines. Most type F FP strains carry a chromosomal <i>cpe</i> gene (c-<i>cpe</i> strains). C. perfringens produces up to three different sialidases, named NanH, NanI, and NanJ, but some c-<i>cpe</i> FP strains carry only <i>nanJ</i> and <i>nanH</i> genes. This study surveyed a collection of such strains and showed that they produce sialidase activity when cultured in Todd-Hewitt broth (TH) (vegetative cultures) or modified Duncan-Strong (MDS) medium (sporulating cultures). Sialidase null mutants were constructed in 01E809, a type F c-<i>cpe</i> FP strain carrying the <i>nanJ</i> and <i>nanH</i> genes. Characterization of those mutants identified NanJ ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-04T20:37:31.012Z","creation":"2025-04-04T20:37:31.012Z"},"accession":"S-EPMC10269042","cross_references":{"pubmed":["37212696"],"doi":["10.1128/iai.00053-23"]}}