biostudies-literatureLi JNIAID NIH HHSHHS | NIH | National Institute of Allergy and Infectious Diseasese0005323https://www.ebi.ac.uk/biostudies/studies/S-EPMC10269042biostudies-literatureUnknown91(6)Clostridium perfringens type F strains cause food poisoning (FP) when they sporulate and produce C. perfringens enterotoxin (CPE) in the intestines. Most type F FP strains carry a chromosomal <i>cpe</i> gene (c-<i>cpe</i> strains). C. perfringens produces up to three different sialidases, named NanH, NanI, and NanJ, but some c-<i>cpe</i> FP strains carry only <i>nanJ</i> and <i>nanH</i> genes. This study surveyed a collection of such strains and showed that they produce sialidase activity when cultured in Todd-Hewitt broth (TH) (vegetative cultures) or modified Duncan-Strong (MDS) medium (sporulating cultures). Sialidase null mutants were constructed in 01E809, a type F c-<i>cpe</i> FP strain carrying the <i>nanJ</i> and <i>nanH</i> genes. Characterization of those mutants identified NanJ Infection and immunityNanJ Is the Major Sialidase for Clostridium perfringens Type F Food Poisoning Strain 01E809.PMC10269042R01AI019844R21 AI146492R21AI146492R01 AI019844Pradhan ALi JMcClane BAfalseNanJ Is the Major Sialidase for Clostridium perfringens Type F Food Poisoning Strain 01E809.Clostridium perfringens type F strains cause food poisoning (FP) when they sporulate and produce C. perfringens enterotoxin (CPE) in the intestines. Most type F FP strains carry a chromosomal <i>cpe</i> gene (c-<i>cpe</i> strains). C. perfringens produces up to three different sialidases, named NanH, NanI, and NanJ, but some c-<i>cpe</i> FP strains carry only <i>nanJ</i> and <i>nanH</i> genes. This study surveyed a collection of such strains and showed that they produce sialidase activity when cultured in Todd-Hewitt broth (TH) (vegetative cultures) or modified Duncan-Strong (MDS) medium (sporulating cultures). Sialidase null mutants were constructed in 01E809, a type F c-<i>cpe</i> FP strain carrying the <i>nanJ</i> and <i>nanH</i> genes. Characterization of those mutants identified NanJ 2023-01-01T00:00:00Z2023 Jun2025-04-04T20:37:31.012Z2025-04-04T20:37:31.012ZS-EPMC102690423721269610.1128/iai.00053-23