{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Paulson AR"],"funding":["Government of Ontario","Government of Canada"],"pagination":["e0140423"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10269869"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(3)"],"pubmed_abstract":["Ticks in the family <i>Ixodidae</i> are important vectors of zoonoses, including Lyme disease (LD), which is caused by spirochete bacteria from the Borreliella (Borrelia) burgdorferi <i>sensu lato</i> complex. The blacklegged tick (Ixodes scapularis) continues to expand across Canada, creating hot spots of elevated LD risk at the leading edge of its expanding range. Current efforts to understand the risk of pathogen transmission associated with I. scapularis in Canada focus primarily on targeted screens, while natural variation in the tick microbiome remains poorly understood. Using multiomics consisting of 16S metabarcoding and ribosome-depleted, whole-shotgun RNA transcriptome sequencing, we examined the microbial communities associated with adult I. scapularis (<i>n</i> = 32), sampled f"],"journal":["Microbiology spectrum"],"pubmed_title":["Multiomics Reveals Symbionts, Pathogens, and Tissue-Specific Microbiome of Blacklegged Ticks (Ixodes scapularis) from a Lyme Disease Hot Spot in Southeastern Ontario, Canada."],"pmcid":["PMC10269869"],"funding_grant_id":["New Frontiers in Research Fund Exploration Grant","Early Researcher Award"],"pubmed_authors":["Paulson AR","Huang D","Colautti RI","Lougheed SC"],"additional_accession":[]},"is_claimable":false,"name":"Multiomics Reveals Symbionts, Pathogens, and Tissue-Specific Microbiome of Blacklegged Ticks (Ixodes scapularis) from a Lyme Disease Hot Spot in Southeastern Ontario, Canada.","description":"Ticks in the family <i>Ixodidae</i> are important vectors of zoonoses, including Lyme disease (LD), which is caused by spirochete bacteria from the Borreliella (Borrelia) burgdorferi <i>sensu lato</i> complex. The blacklegged tick (Ixodes scapularis) continues to expand across Canada, creating hot spots of elevated LD risk at the leading edge of its expanding range. Current efforts to understand the risk of pathogen transmission associated with I. scapularis in Canada focus primarily on targeted screens, while natural variation in the tick microbiome remains poorly understood. Using multiomics consisting of 16S metabarcoding and ribosome-depleted, whole-shotgun RNA transcriptome sequencing, we examined the microbial communities associated with adult I. scapularis (<i>n</i> = 32), sampled f","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-05-28T18:43:53.305Z","creation":"2025-04-03T22:51:05.019Z"},"accession":"S-EPMC10269869","cross_references":{"pubmed":["37184407"],"doi":["10.1128/spectrum.01404-23"]}}