{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["37(9-10)"],"submitter":["Yu AD"],"pubmed_abstract":["A wide range of sequencing methods has been developed to assess nascent RNA transcription and resolve the single-nucleotide position of RNA polymerase genome-wide. These techniques are often burdened with high input material requirements and lengthy protocols. We leveraged the template-switching properties of thermostable group II intron reverse transcriptase (TGIRT) and developed Butt-seq (bulk analysis of nascent transcript termini sequencing), which can produce libraries from purified nascent RNA in 6 h and from as few as 10,000 cells-an improvement of at least 10-fold over existing techniques. Butt-seq shows that inhibition of the superelongation complex (SEC) causes promoter-proximal pausing to move upstream in a fashion correlated with subnucleosomal fragments. To address transcripti"],"journal":["Genes & development"],"pagination":["432-448"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10270195"],"repository":["biostudies-literature"],"pubmed_title":["Butt-seq: a new method for facile profiling of transcription."],"pmcid":["PMC10270195"],"pubmed_authors":["Rosbash M","Yu AD"],"additional_accession":[]},"is_claimable":false,"name":"Butt-seq: a new method for facile profiling of transcription.","description":"A wide range of sequencing methods has been developed to assess nascent RNA transcription and resolve the single-nucleotide position of RNA polymerase genome-wide. These techniques are often burdened with high input material requirements and lengthy protocols. We leveraged the template-switching properties of thermostable group II intron reverse transcriptase (TGIRT) and developed Butt-seq (bulk analysis of nascent transcript termini sequencing), which can produce libraries from purified nascent RNA in 6 h and from as few as 10,000 cells-an improvement of at least 10-fold over existing techniques. Butt-seq shows that inhibition of the superelongation complex (SEC) causes promoter-proximal pausing to move upstream in a fashion correlated with subnucleosomal fragments. To address transcripti","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2026-05-28T12:10:28.24Z","creation":"2025-02-19T00:50:12.483Z"},"accession":"S-EPMC10270195","cross_references":{"pubmed":["37164645"],"doi":["10.1101/gad.350434.123"]}}