{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ju X"],"funding":["Beijing Municipal Natural Science Foundation","2030 Innovation Tsinghua Initiative","National Natural Science Foundation of China","Shuimu Tsinghua Scholar Program","The Postdoctoral Science Foundation of China"],"pagination":["e1011434"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10270597"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(6)"],"pubmed_abstract":["Hepatitis E virus (HEV) is one of the main pathogenic agents of acute hepatitis in the world. The mechanism of HEV replication, especially host factors governing HEV replication is still not clear. Here, using HEV ORF1 trans-complementation cell culture system and HEV replicon system, combining with stable isotope labelling with amino acids in cell culture (SILAC) and mass spectrometry (MS), we aimed to identify the host factors regulating HEV replication. We identified a diversity of host factors associated with HEV ORF1 protein, which were putatively responsible for viral genomic RNA replication, in these two cell culture models. Of note, the protein arginine methyltransferase 5 (PRMT5)/WDR77 complex was identified in both cell culture models as the top hit. Furthermore, we demonstrated "],"journal":["PLoS pathogens"],"pubmed_title":["The PRMT5/WDR77 complex restricts hepatitis E virus replication."],"pmcid":["PMC10270597"],"funding_grant_id":["2021SM107","82241077","82272302","2021TQ0182","Z220018","32070153","32200111","100021067"],"pubmed_authors":["Ju X","Ren W","Dong L","Ding Q","Nan Y","Meng X","Deng H","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"The PRMT5/WDR77 complex restricts hepatitis E virus replication.","description":"Hepatitis E virus (HEV) is one of the main pathogenic agents of acute hepatitis in the world. The mechanism of HEV replication, especially host factors governing HEV replication is still not clear. Here, using HEV ORF1 trans-complementation cell culture system and HEV replicon system, combining with stable isotope labelling with amino acids in cell culture (SILAC) and mass spectrometry (MS), we aimed to identify the host factors regulating HEV replication. We identified a diversity of host factors associated with HEV ORF1 protein, which were putatively responsible for viral genomic RNA replication, in these two cell culture models. Of note, the protein arginine methyltransferase 5 (PRMT5)/WDR77 complex was identified in both cell culture models as the top hit. Furthermore, we demonstrated ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-06-25T03:15:27.279Z","creation":"2025-04-06T02:35:13.339Z"},"accession":"S-EPMC10270597","cross_references":{"pubmed":["37276230"],"doi":["10.1371/journal.ppat.1011434"]}}