biostudies-literatureHouston JNational Research Foundation of KoreaNational Institutes of HealthNational Institute of General Medical SciencesLudwig Institute for Cancer ResearchNIGMS NIH HHSNational Science Foundation2291-2299.e10https://www.ebi.ac.uk/biostudies/studies/S-EPMC10270731biostudies-literatureUnknown33(11)During mitosis, chromosomes assemble kinetochores to dynamically couple with spindle microtubules.¹^,² Kinetochores also function as signaling hubs directing mitotic progression by recruiting and controlling the fate of the anaphase promoting complex/cyclosome (APC/C) activator CDC-20.³^,⁴^,⁵ Kinetochores either incorporate CDC-20 into checkpoint complexes that inhibit the APC/C or dephosphorylate CDC-20, which allows it to interact with and activate the APC/C.⁴^,⁶ The importance of these two CDC-20 fates likely depends on the biological context. In human somatic cells, the major mechanism controlling mitotic progression is the spindle checkpoint. By contrast, progression thCurrent biology : CBBUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses.PMC102707311650112R01 GM074215NRF-2020RC1C1008596R35 GM144121R01 GM074207Kim TOegema KGomez-Cavazos JSDeep AHouston JDesai ALara-Gonzalez POhta MCorbett KDfalseBUB-1-bound PLK-1 directs CDC-20 kinetochore recruitment to ensure timely embryonic mitoses.During mitosis, chromosomes assemble kinetochores to dynamically couple with spindle microtubules.¹^,² Kinetochores also function as signaling hubs directing mitotic progression by recruiting and controlling the fate of the anaphase promoting complex/cyclosome (APC/C) activator CDC-20.³^,⁴^,⁵ Kinetochores either incorporate CDC-20 into checkpoint complexes that inhibit the APC/C or dephosphorylate CDC-20, which allows it to interact with and activate the APC/C.⁴^,⁶ The importance of these two CDC-20 fates likely depends on the biological context. In human somatic cells, the major mechanism controlling mitotic progression is the spindle checkpoint. By contrast, progression th2023-01-01T00:00:00Z2023 Jun2026-04-08T19:48:24.322Z2026-04-08T14:33:07.942ZS-EPMC102707313713730810.1016/j.cub.2023.04.021