biostudies-literatureMa POpening Project of Hubei Key Laboratory102https://www.ebi.ac.uk/biostudies/studies/S-EPMC10272048biostudies-literatureUnknown14(1)Intrahepatic cholangiocarcinoma (ICC) is a fatal disease and the molecular mechanism of its progression remains unknown. Aurora Kinase B (AURKB) is a central regulator of chromosome separation and cytokinesis and is abnormally expressed in a variety of cancer cells. This research aimed to explore the effect of AURKB in occurrence and metastasis of ICC. We found that AURKB showed a progressive up-regulation pattern from normal bile duct tissue to ICC with high invasion. Our data showed that AURKB significantly promoted ICC cell proliferation, induced epithelial-mesenchymal transition (EMT), migration and invasion through gain- and loss- of function experiments. In vivo results consistently showed that AURKB up-regulation not only promoted tumor growth, but also promoted tumor metastasis. ImDiscover. OncologyAURKB activates EMT through PI3K/AKT signaling axis to promote ICC progression.PMC102720482021KFY007Ma PZhang YFWang WYu KHHao YWang WXfalseAURKB activates EMT through PI3K/AKT signaling axis to promote ICC progression.Intrahepatic cholangiocarcinoma (ICC) is a fatal disease and the molecular mechanism of its progression remains unknown. Aurora Kinase B (AURKB) is a central regulator of chromosome separation and cytokinesis and is abnormally expressed in a variety of cancer cells. This research aimed to explore the effect of AURKB in occurrence and metastasis of ICC. We found that AURKB showed a progressive up-regulation pattern from normal bile duct tissue to ICC with high invasion. Our data showed that AURKB significantly promoted ICC cell proliferation, induced epithelial-mesenchymal transition (EMT), migration and invasion through gain- and loss- of function experiments. In vivo results consistently showed that AURKB up-regulation not only promoted tumor growth, but also promoted tumor metastasis. Im2023-01-01T00:00:00Z2023 Jun2025-04-22T01:58:02.729Z2025-04-05T20:14:10.822ZS-EPMC102720483731867610.1007/s12672-023-00707-1