biostudies-literatureUnknown14Lim Kam Sian TCCVictorian Cancer AgencyHuman leukocyte antigen (HLA) molecules play a crucial role in directing adaptive immune responses based on the nature of their peptide ligands, collectively coined the immunopeptidome. As such, the study of HLA molecules has been of major interest in the development of cancer immunotherapies such as vaccines and T-cell therapies. Hence, a comprehensive understanding and profiling of the immunopeptidome is required to foster the growth of these personalised solutions. We herein describe SAPrIm, an Immunopeptidomics tool for the Mid-Throughput era. This is a semi-automated workflow involving the KingFisher platform to isolate immunopeptidomes using anti-HLA antibodies coupled to a hyper-porous magnetic protein A microbead, a variable window data independent acquisition (DIA) method and the Frontiers in immunology1107576https://www.ebi.ac.uk/biostudies/studies/S-EPMC10272402biostudies-literatureSAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics.PMC10272402Purcell AWGoncalves GLim Kam Sian TCCShahbazy MJin DShamekhi TSteele JRBramberger LFaridi PStoychev SRamarathinam SfalseSAPrIm, a semi-automated protocol for mid-throughput immunopeptidomics.Human leukocyte antigen (HLA) molecules play a crucial role in directing adaptive immune responses based on the nature of their peptide ligands, collectively coined the immunopeptidome. As such, the study of HLA molecules has been of major interest in the development of cancer immunotherapies such as vaccines and T-cell therapies. Hence, a comprehensive understanding and profiling of the immunopeptidome is required to foster the growth of these personalised solutions. We herein describe SAPrIm, an Immunopeptidomics tool for the Mid-Throughput era. This is a semi-automated workflow involving the KingFisher platform to isolate immunopeptidomes using anti-HLA antibodies coupled to a hyper-porous magnetic protein A microbead, a variable window data independent acquisition (DIA) method and the 2023-01-01T00:00:00Z20232026-05-13T14:26:04.722Z2025-04-03T22:50:46.77ZS-EPMC102724023733436510.3389/fimmu.2023.1107576