{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["7(6)"],"submitter":["Che L"],"pubmed_abstract":["Cyanobacterial NdhM, an oxygenic photosynthesis-specific NDH-1 subunit, has been found to be essential for the formation of a large complex of NDH-1 (NDH-1L). The cryo-electron microscopic (cryo-EM) structure of NdhM from <i>Thermosynechococcus elongatus</i> showed that the N-terminus of NdhM contains three β-sheets, while two α-helixes are present in the middle and C-terminal part of NdhM. Here, we obtained a mutant of the unicellular cyanobacterium <i>Synechocystis</i> 6803 expressing a C-terminal truncated NdhM subunit designated NdhMΔC. Accumulation and activity of NDH-1 were not affected in NdhMΔC under normal growth conditions. However, the NDH-1 complex with truncated NdhM is unstable under stress. Immunoblot analyses showed that the assembly process of the cyanobacterial NDH-1L hyd"],"journal":["Plant direct"],"pagination":["e502"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10272980"],"repository":["biostudies-literature"],"pubmed_title":["NDH-1L with a truncated NdhM subunit is unstable under stress conditions in cyanobacteria."],"pmcid":["PMC10272980"],"pubmed_authors":["Huang Y","Guo Y","Che L","Peng L","Gao F"],"additional_accession":[]},"is_claimable":false,"name":"NDH-1L with a truncated NdhM subunit is unstable under stress conditions in cyanobacteria.","description":"Cyanobacterial NdhM, an oxygenic photosynthesis-specific NDH-1 subunit, has been found to be essential for the formation of a large complex of NDH-1 (NDH-1L). The cryo-electron microscopic (cryo-EM) structure of NdhM from <i>Thermosynechococcus elongatus</i> showed that the N-terminus of NdhM contains three β-sheets, while two α-helixes are present in the middle and C-terminal part of NdhM. Here, we obtained a mutant of the unicellular cyanobacterium <i>Synechocystis</i> 6803 expressing a C-terminal truncated NdhM subunit designated NdhMΔC. Accumulation and activity of NDH-1 were not affected in NdhMΔC under normal growth conditions. However, the NDH-1 complex with truncated NdhM is unstable under stress. Immunoblot analyses showed that the assembly process of the cyanobacterial NDH-1L hyd","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-04-29T02:28:31.178Z","creation":"2025-02-19T04:01:43.24Z"},"accession":"S-EPMC10272980","cross_references":{"pubmed":["37334271"],"doi":["10.1002/pld3.502"]}}