{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["25(1)"],"submitter":["Wilson TG"],"funding":["Henry Ford Health"],"pubmed_abstract":["<h4>Objective</h4>We aimed to characterize the expression patterns, gene targets, and functional effects of miR-335-5p and miR-335-3p among seven primary human knee and hip osteoarthritic tissue types.<h4>Methods</h4>We collected synovial fluid, subchondral bone, articular cartilage, synovium, meniscus/labrum, infrapatellar/acetabular fat, anterior cruciate ligament/ligamentum teres, and vastus medialis oblique/quadratus femoris muscle (n = 7-20) from surgical patients with early- or late-stage osteoarthritis (OA) and quantified miR-335-5p and miR-335-3p expression by real-time PCR. Predicted gene targets were measured in knee OA infrapatellar fat following miRNA inhibitor transfection (n = 3), and prioritized gene targets were validated following miRNA inhibitor and mimic transfection (n "],"journal":["Arthritis research & therapy"],"pagination":["105"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10273720"],"repository":["biostudies-literature"],"pubmed_title":["Characterization of miR-335-5p and miR-335-3p in human osteoarthritic tissues."],"pmcid":["PMC10273720"],"pubmed_authors":["Wilson TG","Baghel M","Moutzouros V","Davis J","Kaur N","Ali SA"],"additional_accession":[]},"is_claimable":false,"name":"Characterization of miR-335-5p and miR-335-3p in human osteoarthritic tissues.","description":"<h4>Objective</h4>We aimed to characterize the expression patterns, gene targets, and functional effects of miR-335-5p and miR-335-3p among seven primary human knee and hip osteoarthritic tissue types.<h4>Methods</h4>We collected synovial fluid, subchondral bone, articular cartilage, synovium, meniscus/labrum, infrapatellar/acetabular fat, anterior cruciate ligament/ligamentum teres, and vastus medialis oblique/quadratus femoris muscle (n = 7-20) from surgical patients with early- or late-stage osteoarthritis (OA) and quantified miR-335-5p and miR-335-3p expression by real-time PCR. Predicted gene targets were measured in knee OA infrapatellar fat following miRNA inhibitor transfection (n = 3), and prioritized gene targets were validated following miRNA inhibitor and mimic transfection (n ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-04T07:38:00.02Z","creation":"2025-04-04T07:38:00.02Z"},"accession":"S-EPMC10273720","cross_references":{"pubmed":["37328905"],"doi":["10.1186/s13075-023-03088-6"]}}