biostudies-literatureUnknown25(1)Wilson TGHenry Ford Health<h4>Objective</h4>We aimed to characterize the expression patterns, gene targets, and functional effects of miR-335-5p and miR-335-3p among seven primary human knee and hip osteoarthritic tissue types.<h4>Methods</h4>We collected synovial fluid, subchondral bone, articular cartilage, synovium, meniscus/labrum, infrapatellar/acetabular fat, anterior cruciate ligament/ligamentum teres, and vastus medialis oblique/quadratus femoris muscle (n = 7-20) from surgical patients with early- or late-stage osteoarthritis (OA) and quantified miR-335-5p and miR-335-3p expression by real-time PCR. Predicted gene targets were measured in knee OA infrapatellar fat following miRNA inhibitor transfection (n = 3), and prioritized gene targets were validated following miRNA inhibitor and mimic transfection (n Arthritis research & therapy105https://www.ebi.ac.uk/biostudies/studies/S-EPMC10273720biostudies-literatureCharacterization of miR-335-5p and miR-335-3p in human osteoarthritic tissues.PMC10273720Wilson TGBaghel MMoutzouros VDavis JKaur NAli SAfalseCharacterization of miR-335-5p and miR-335-3p in human osteoarthritic tissues.<h4>Objective</h4>We aimed to characterize the expression patterns, gene targets, and functional effects of miR-335-5p and miR-335-3p among seven primary human knee and hip osteoarthritic tissue types.<h4>Methods</h4>We collected synovial fluid, subchondral bone, articular cartilage, synovium, meniscus/labrum, infrapatellar/acetabular fat, anterior cruciate ligament/ligamentum teres, and vastus medialis oblique/quadratus femoris muscle (n = 7-20) from surgical patients with early- or late-stage osteoarthritis (OA) and quantified miR-335-5p and miR-335-3p expression by real-time PCR. Predicted gene targets were measured in knee OA infrapatellar fat following miRNA inhibitor transfection (n = 3), and prioritized gene targets were validated following miRNA inhibitor and mimic transfection (n 2023-01-01T00:00:00Z2023 Jun2025-04-04T07:38:00.02Z2025-04-04T07:38:00.02ZS-EPMC102737203732890510.1186/s13075-023-03088-6