{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Tsissios G"],"funding":["NEI NIH HHS","NINDS NIH HHS"],"pubmed_abstract":["Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be visualized <i>in vivo</i>. Using this new tool, we analyzed the location of macrophages during lens regeneration. We uncovered early gene expression changes using bulk RNAseq in two newt species, <i>Notophthalmus viridescens</i> and <i>Pleurodeles waltl</i>. Next, we used clodronate liposomes to deplete macrophages, which inhibited lens regeneration in both newt species. Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increa"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.06.04.543633"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10274724"],"repository":["biostudies-literature"],"pubmed_title":["Macrophages modulate fibrosis during newt lens regeneration."],"pmcid":["PMC10274724"],"funding_grant_id":["R21 EY031865","R01 EY027801","R21 EY033916","F99 NS129167"],"pubmed_authors":["Tangeman JA","Araus AJ","Ratvasky SC","Grajales-Esquivel E","Wang H","Yun MH","Simon A","Tsissios G","Perez-Estrada JR","Rio-Tsonis KD","Chen W","Sallese A","Martinez A","Visser KJ","Smucker B"],"additional_accession":[]},"is_claimable":false,"name":"Macrophages modulate fibrosis during newt lens regeneration.","description":"Previous studies indicated that macrophages play a role during lens regeneration in newts, but their function has not been tested experimentally. Here we generated a transgenic newt reporter line in which macrophages can be visualized <i>in vivo</i>. Using this new tool, we analyzed the location of macrophages during lens regeneration. We uncovered early gene expression changes using bulk RNAseq in two newt species, <i>Notophthalmus viridescens</i> and <i>Pleurodeles waltl</i>. Next, we used clodronate liposomes to deplete macrophages, which inhibited lens regeneration in both newt species. Macrophage depletion induced the formation of scar-like tissue, an increased and sustained inflammatory response, an early decrease in iris pigment epithelial cell (iPEC) proliferation and a late increa","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-18T19:23:08.803Z","creation":"2025-04-07T07:07:37.487Z"},"accession":"S-EPMC10274724","cross_references":{"pubmed":["37333184"],"doi":["10.1101/2023.06.04.543633"]}}