{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Acevedo-Sanchez Y"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Interorganelle communication regulates cellular homeostasis through the formation of tightly-associated membrane contact sites <sup>1-3</sup>. Prior work has identified several ways that intracellular pathogens alter contacts between eukaryotic membranes <sup>4-6</sup>, but there is no existing evidence for contact sites spanning eukaryotic and prokaryotic membranes. Here, using a combination of live-cell microscopy and transmission and focused-ion-beam scanning electron microscopy, we demonstrate that the intracellular bacterial pathogen <i>Rickettsia parkeri</i> forms a direct membrane contact site between its bacterial outer membrane and the rough endoplasmic reticulum (ER), with tethers that are approximately 55 nm apart. Depletion of the ER-specific tethers VAPA and VAPB reduced the f"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.06.05.543771"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10274737"],"repository":["biostudies-literature"],"pubmed_title":["An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site."],"pmcid":["PMC10274737"],"funding_grant_id":["R01 AI155489","R01 GM141025","T32 GM007287"],"pubmed_authors":["Acevedo-Sanchez Y","Lamason RL","Woida PJ","Kraemer S"],"additional_accession":[]},"is_claimable":false,"name":"An obligate intracellular bacterial pathogen forms a direct, interkingdom membrane contact site.","description":"Interorganelle communication regulates cellular homeostasis through the formation of tightly-associated membrane contact sites <sup>1-3</sup>. Prior work has identified several ways that intracellular pathogens alter contacts between eukaryotic membranes <sup>4-6</sup>, but there is no existing evidence for contact sites spanning eukaryotic and prokaryotic membranes. Here, using a combination of live-cell microscopy and transmission and focused-ion-beam scanning electron microscopy, we demonstrate that the intracellular bacterial pathogen <i>Rickettsia parkeri</i> forms a direct membrane contact site between its bacterial outer membrane and the rough endoplasmic reticulum (ER), with tethers that are approximately 55 nm apart. Depletion of the ER-specific tethers VAPA and VAPB reduced the f","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-06-22T03:17:31.684Z","creation":"2025-04-06T12:23:14.3Z"},"accession":"S-EPMC10274737","cross_references":{"pubmed":["37333133"],"doi":["10.1101/2023.06.05.543771"]}}