biostudies-literatureUnknownMaggiore JCNIBIB NIH HHSNIDDK NIH HHSNHLBI NIH HHSNIH HHSVascularization plays a critical role in organ maturation and cell type development. Drug discovery, organ mimicry, and ultimately transplantation in a clinical setting thereby hinges on achieving robust vascularization of <i>in vitro</i> engineered organs. Here, focusing on human kidney organoids, we overcome this hurdle by combining an inducible <i>ETS translocation variant 2</i> (<i>ETV2</i>) human induced pluripotent stem cell (iPSC) line, which directs endothelial fate, with a non-transgenic iPSC line in suspension organoid culture. The resulting human kidney organoids show extensive vascularization by endothelial cells with an identity most closely related to endogenous kidney endothelia. Vascularized organoids also show increased maturation of nephron structures including more maturbioRxiv : the preprint server for biology2023.05.30.542848https://www.ebi.ac.uk/biostudies/studies/S-EPMC10274893biostudies-literatureGenetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.PMC10274893R01 HL141805R56 DK125960R01 DK127634P30 DK079307RC2 DK125960UC2 DK126122R01 EB024562R01 EB028532S10 OD019973T32 EB001026Maggiore JCCarroll TJLeGraw RLittle MWu HSims-Lucas SVelazquez JPfister KWatson AHill APrzepiorski ASilva-Barbosa AHislop JStolz DBStreeter EEbrahimkhani MRChaney CFranks JHumphreys BDWatkins SCHowden SEHukriede NAKiani SCleaver ODavidson AJfalseGenetically engineering endothelial niche in human kidney organoids enables multilineage maturation, vascularization and de novo cell types.Vascularization plays a critical role in organ maturation and cell type development. Drug discovery, organ mimicry, and ultimately transplantation in a clinical setting thereby hinges on achieving robust vascularization of <i>in vitro</i> engineered organs. Here, focusing on human kidney organoids, we overcome this hurdle by combining an inducible <i>ETS translocation variant 2</i> (<i>ETV2</i>) human induced pluripotent stem cell (iPSC) line, which directs endothelial fate, with a non-transgenic iPSC line in suspension organoid culture. The resulting human kidney organoids show extensive vascularization by endothelial cells with an identity most closely related to endogenous kidney endothelia. Vascularized organoids also show increased maturation of nephron structures including more matur2023-01-01T00:00:00Z2023 May2026-06-22T03:22:21.634Z2026-06-22T03:09:06.323ZS-EPMC102748933733315510.1101/2023.05.30.542848