{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(24)"],"submitter":["Ng JWK"],"pubmed_abstract":["Human cord blood-derived γδ T cells (CB<sub>γδ</sub>) display a highly diverse TCR<sub>γδ</sub> repertoire and have a unique subtype composition different from fetal or adult peripheral blood counterparts. We expanded CB<sub>γδ</sub> in vitro using an irradiated Epstein-Barr virus-transformed feeder cell-based modified rapid expansion protocol (REP). Single-cell RNA sequencing tracked progressive differentiation of naïve CB<sub>γδ</sub> into cells expressing neoantigen-reactive tumor-infiltrating lymphocyte as well as tissue-resident memory precursor-like and antigen-presenting cell-like gene signatures. TCR<sub>γδ</sub> clonal tracing revealed a bias toward cytotoxic effector differentiation in a much larger proportion of V<sub>δ</sub>2<sup>-</sup> clones compared to V<sub>δ</sub>2<sup>+<"],"journal":["Science advances"],"pagination":["eadf3120"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10275585"],"repository":["biostudies-literature"],"pubmed_title":["Cord blood-derived V<sub>δ</sub>2<sup>+</sup> and V<sub>δ</sub>2<sup>-</sup> T cells acquire differential cell state compositions upon in vitro expansion."],"pmcid":["PMC10275585"],"pubmed_authors":["Ng JWK","Fan X","Hwang WYK","Lim AST","Lai JJH","Tan KW","Poon Z","Lim TH","Goh YT","Cheung AMS","Eaves CJ","Li S","Lim TKH","Guo DY"],"additional_accession":[]},"is_claimable":false,"name":"Cord blood-derived V<sub>δ</sub>2<sup>+</sup> and V<sub>δ</sub>2<sup>-</sup> T cells acquire differential cell state compositions upon in vitro expansion.","description":"Human cord blood-derived γδ T cells (CB<sub>γδ</sub>) display a highly diverse TCR<sub>γδ</sub> repertoire and have a unique subtype composition different from fetal or adult peripheral blood counterparts. We expanded CB<sub>γδ</sub> in vitro using an irradiated Epstein-Barr virus-transformed feeder cell-based modified rapid expansion protocol (REP). Single-cell RNA sequencing tracked progressive differentiation of naïve CB<sub>γδ</sub> into cells expressing neoantigen-reactive tumor-infiltrating lymphocyte as well as tissue-resident memory precursor-like and antigen-presenting cell-like gene signatures. TCR<sub>γδ</sub> clonal tracing revealed a bias toward cytotoxic effector differentiation in a much larger proportion of V<sub>δ</sub>2<sup>-</sup> clones compared to V<sub>δ</sub>2<sup>+<","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-07-02T03:04:36.914Z","creation":"2025-07-02T03:04:36.914Z"},"accession":"S-EPMC10275585","cross_references":{"pubmed":["37327346"],"doi":["10.1126/sciadv.adf3120"]}}