{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["9(24)"],"submitter":["Wang F"],"pubmed_abstract":["In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45<sup>-</sup> nonimmune cells and 196,473 CD45<sup>+</sup> immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. <i>F3</i><sup>+</sup> fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, <i>MCAM</i><sup>+</sup> fib"],"journal":["Science advances"],"pagination":["eadf5464"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10275599"],"repository":["biostudies-literature"],"pubmed_title":["Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer."],"pmcid":["PMC10275599"],"pubmed_authors":["Huang C","Long J","Cao J","Wang F","Da TT","Li L","Wang XQ","Yao XQ","Lian ZX","Jiang YH","Ma HQ","Zhao ZB","Wu ZX"],"additional_accession":[]},"is_claimable":false,"name":"Single-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer.","description":"In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45<sup>-</sup> nonimmune cells and 196,473 CD45<sup>+</sup> immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. <i>F3</i><sup>+</sup> fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, <i>MCAM</i><sup>+</sup> fib","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-05-28T18:43:50.41Z","creation":"2025-04-03T22:51:03.928Z"},"accession":"S-EPMC10275599","cross_references":{"pubmed":["37327339"],"doi":["10.1126/sciadv.adf5464"]}}