biostudies-literatureUnknown9(24)Wang FIn this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45^- nonimmune cells and 196,473 CD45⁺ immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. <i>F3</i>⁺ fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, <i>MCAM</i>⁺ fibScience advanceseadf5464https://www.ebi.ac.uk/biostudies/studies/S-EPMC10275599biostudies-literatureSingle-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer.PMC10275599Huang CLong JCao JWang FDa TTLi LWang XQYao XQLian ZXJiang YHMa HQZhao ZBWu ZXfalseSingle-cell and spatial transcriptome analysis reveals the cellular heterogeneity of liver metastatic colorectal cancer.In this study, we comprehensively charted the cellular landscape of colorectal cancer (CRC) and well-matched liver metastatic CRC using single-cell and spatial transcriptome RNA sequencing. We generated 41,892 CD45^- nonimmune cells and 196,473 CD45⁺ immune cells from 27 samples of six CRC patients, and found that CD8_CXCL13 and CD4_CXCL13 subsets increased significantly in liver metastatic samples that exhibited high proliferation ability and tumor-activating characterization, contributing to better prognosis of patients. Distinct fibroblast profiles were observed in primary and liver metastatic tumors. <i>F3</i>⁺ fibroblasts enriched in primary tumors contributed to worse overall survival by expressing protumor factors. However, <i>MCAM</i>⁺ fib2023-01-01T00:00:00Z2023 Jun2026-05-28T18:43:50.41Z2025-04-03T22:51:03.928ZS-EPMC102755993732733910.1126/sciadv.adf5464