biostudies-literatureKannan ANIEHS NIH HHSNCI NIH HHSNational Institutes of Health503440https://www.ebi.ac.uk/biostudies/studies/S-EPMC10276974biostudies-literatureUnknown874-875CYP1B1 activates many chemical carcinogens into potent genotoxins, and allelic variants are risk factors in lung, breast, and prostate cancer. However, few eukaryotic genetic instability endpoints have been directly measured for CYP1B1-activated metabolites. In this study, we expressed human CYP1B1 in yeast strains that measure DNA damage-associated toxicity and frequencies of chromosomal translocations. DNA damage-associated toxicity was measured in a rad4 rad51 strain, defective in both DNA excision and recombinational repair. Frequencies of chromosomal translocations were measured in diploid yeast strains containing two his3 fragments. These strains were exposed to benzo[a]pyrene-7,8-dihydrodiol (BaP-DHD), aflatoxin B₁ (AFB₁), and the heterocyclic aromatic amines, Mutation research. Genetic toxicology and environmental mutagenesisCYP1B1 converts procarcinogens into genotoxins in Saccharomyces cerevisiae.PMC10276974R15ES023685-01R21 ES015954R15 ES023685R21 CA125064R21ES015954Kannan APerpetua NDolan MFasullo MfalseCYP1B1 converts procarcinogens into genotoxins in Saccharomyces cerevisiae.CYP1B1 activates many chemical carcinogens into potent genotoxins, and allelic variants are risk factors in lung, breast, and prostate cancer. However, few eukaryotic genetic instability endpoints have been directly measured for CYP1B1-activated metabolites. In this study, we expressed human CYP1B1 in yeast strains that measure DNA damage-associated toxicity and frequencies of chromosomal translocations. DNA damage-associated toxicity was measured in a rad4 rad51 strain, defective in both DNA excision and recombinational repair. Frequencies of chromosomal translocations were measured in diploid yeast strains containing two his3 fragments. These strains were exposed to benzo[a]pyrene-7,8-dihydrodiol (BaP-DHD), aflatoxin B₁ (AFB₁), and the heterocyclic aromatic amines, 2022-01-01T00:00:00Z2022 Feb-Mar2025-04-22T01:59:11.15Z2024-11-05T20:31:02.158ZS-EPMC102769743515142310.1016/j.mrgentox.2022.503440