biostudies-literatureUnknown14Wright ML<h4>Background</h4>Pregnancy is known to induce extensive biological changes in the healthy mother. Little is known, however, about what these changes are at the molecular level. We have examined systemic expression changes in protein-coding genes and long non-coding (lnc) RNAs during and after pregnancy, compared to before pregnancy, among healthy women with term pregnancies.<h4>Methods</h4>Blood samples were collected from 14 healthy women enrolled in our prospective pregnancy cohort at 7 time-points (before, during and after pregnancy). Total RNA from frozen whole blood was used for RNA sequencing. Following raw read alignment and assembly, gene-level counts were obtained for protein-coding genes and long non-coding RNAs. At each time-point, cell type proportions were estimated using deFrontiers in immunology1161084https://www.ebi.ac.uk/biostudies/studies/S-EPMC10277629biostudies-literaturePregnancy-associated systemic gene expression compared to a pre-pregnancy baseline, among healthy women with term pregnancies.PMC10277629Olsen JZoffmann VSmed MKJewell NPNelson JLJawaheer DGoin DEWright MLHetland MLfalsePregnancy-associated systemic gene expression compared to a pre-pregnancy baseline, among healthy women with term pregnancies.<h4>Background</h4>Pregnancy is known to induce extensive biological changes in the healthy mother. Little is known, however, about what these changes are at the molecular level. We have examined systemic expression changes in protein-coding genes and long non-coding (lnc) RNAs during and after pregnancy, compared to before pregnancy, among healthy women with term pregnancies.<h4>Methods</h4>Blood samples were collected from 14 healthy women enrolled in our prospective pregnancy cohort at 7 time-points (before, during and after pregnancy). Total RNA from frozen whole blood was used for RNA sequencing. Following raw read alignment and assembly, gene-level counts were obtained for protein-coding genes and long non-coding RNAs. At each time-point, cell type proportions were estimated using de2023-01-01T00:00:00Z20232025-04-26T09:03:12.135Z2025-04-06T12:56:20.083ZS-EPMC102776293734234910.3389/fimmu.2023.1161084