biostudies-literatureYuan XQFundamental Research Funds for the Central UniversitiesBasic and Applied Basic Research Foundation of Guangdong ProvinceGuangdong Provincial Natural Science FoundationNational Natural Science Foundation of ChinaChina Postdoctoral Science FoundationNational Postdoctoral Program for Innovative Talents1756-1774https://www.ebi.ac.uk/biostudies/studies/S-EPMC10277835biostudies-literatureUnknown31(6)Super-enhancer (SE) plays a vital role in the determination of cell identity and fate. Up-regulated expression of coding genes is frequently associated with SE. However, the transcription dysregulation driven by SE, from the viewpoint of long non-coding RNA (lncRNA), remains unclear. Here, SE-associated lncRNAs in HCC are comprehensively outlined for the first time. This study integrally screens and identifies several novel SE-associated lncRNAs that are highly abundant and sensitive to JQ1. Especially, HSAL3 is identified as an uncharacterized SE-driven oncogenic lncRNA, which is activated by transcription factors HCFC1 and HSF1 via its super-enhancer. HSAL3 interference negatively regulates NOTCH signaling, implying the potential mechanism of its tumor-promoting role. The expression of HMolecular therapy : the journal of the American Society of Gene TherapyAnchoring super-enhancer-driven oncogenic lncRNAs for anti-tumor therapy in hepatocellular carcinoma.PMC102778352019A1515012114818028122019M6632542018A030313129BX2019039581803636819726582018A030313032920ykpy105Li GCWang JPZhou NWang SYuan XQPeng LYang XZZhang CYfalseAnchoring super-enhancer-driven oncogenic lncRNAs for anti-tumor therapy in hepatocellular carcinoma.Super-enhancer (SE) plays a vital role in the determination of cell identity and fate. Up-regulated expression of coding genes is frequently associated with SE. However, the transcription dysregulation driven by SE, from the viewpoint of long non-coding RNA (lncRNA), remains unclear. Here, SE-associated lncRNAs in HCC are comprehensively outlined for the first time. This study integrally screens and identifies several novel SE-associated lncRNAs that are highly abundant and sensitive to JQ1. Especially, HSAL3 is identified as an uncharacterized SE-driven oncogenic lncRNA, which is activated by transcription factors HCFC1 and HSF1 via its super-enhancer. HSAL3 interference negatively regulates NOTCH signaling, implying the potential mechanism of its tumor-promoting role. The expression of H2023-01-01T00:00:00Z2023 Jun2026-06-01T10:59:10.924Z2026-04-08T11:40:23.232ZS-EPMC102778353646163310.1016/j.ymthe.2022.11.013