biostudies-literatureUnknown31(6)Xu XN⁶-Methyladenosine (m⁶A) RNA modification, methylation at the N6 position of adenosine, plays critical roles in tumorigenesis. m⁶A readers recognize m⁶A modifications and thus act as key executors for the biological consequences of RNA methylation. However, knowledge about the regulatory mechanism(s) of m⁶A readers is extremely limited. In this study, RN7SK was identified as a small nuclear RNA that interacts with m⁶A readers. m⁶A readers recognized and facilitated secondary structure formation of m⁶A-modified RN7SK, which in turn prevented m⁶A reader mRNA degradation from exonucleases. Thus, a positive feedback circuit between RN7SK and m⁶A readers is established in tumor cells. FMolecular therapy : the journal of the American Society of Gene Therapy1615-1635https://www.ebi.ac.uk/biostudies/studies/S-EPMC10277899biostudies-literatureA positive feedback circuit between RN7SK snRNA and m⁶A readers is essential for tumorigenesis.PMC10277899Ma LQiu STian XZhang XGuo WMiao YGuo SXu XWang YWang JYu YfalseA positive feedback circuit between RN7SK snRNA and m⁶A readers is essential for tumorigenesis.N⁶-Methyladenosine (m⁶A) RNA modification, methylation at the N6 position of adenosine, plays critical roles in tumorigenesis. m⁶A readers recognize m⁶A modifications and thus act as key executors for the biological consequences of RNA methylation. However, knowledge about the regulatory mechanism(s) of m⁶A readers is extremely limited. In this study, RN7SK was identified as a small nuclear RNA that interacts with m⁶A readers. m⁶A readers recognized and facilitated secondary structure formation of m⁶A-modified RN7SK, which in turn prevented m⁶A reader mRNA degradation from exonucleases. Thus, a positive feedback circuit between RN7SK and m⁶A readers is established in tumor cells. F2023-01-01T00:00:00Z2023 Jun2026-06-01T10:58:52.188Z2026-04-08T11:39:46.386ZS-EPMC102778993656634910.1016/j.ymthe.2022.12.013