biostudies-literatureUnknown14Xu JBeijing University of Chinese MedicineGuizhou Medical UniversityCurcumin is well known as a potent antioxidant and free radical scavenger and has great potential for anti-aging applications. In this study, we investigate the molecular mechanism of curcumin in prolonging the lifespan of <i>C. elegans</i>. Four concentrations of curcumin (10, 25, 50, and 100 µM) were administered, and the optimal treatment concentration was determined by analyzing the nematode lifespan, physiology, and biochemistry. Additionally, RNA-seq and qRT-PCR were performed to explore the antioxidant effect of curcumin and its underlying mechanism. Results revealed that curcumin could significantly improve the survival capacity of <i>C. elegans</i> without influencing its growth. Curcumin was observed to significantly decrease the levels of reactive oxygen species (ROS) under extrFrontiers in pharmacology1195490https://www.ebi.ac.uk/biostudies/studies/S-EPMC10279890biostudies-literatureCurcumin supplementation increases longevity and antioxidant capacity in <i>Caenorhabditis elegans</i>.PMC10279890Liu XDu PGe YXu XZhang CXu JfalseCurcumin supplementation increases longevity and antioxidant capacity in <i>Caenorhabditis elegans</i>.Curcumin is well known as a potent antioxidant and free radical scavenger and has great potential for anti-aging applications. In this study, we investigate the molecular mechanism of curcumin in prolonging the lifespan of <i>C. elegans</i>. Four concentrations of curcumin (10, 25, 50, and 100 µM) were administered, and the optimal treatment concentration was determined by analyzing the nematode lifespan, physiology, and biochemistry. Additionally, RNA-seq and qRT-PCR were performed to explore the antioxidant effect of curcumin and its underlying mechanism. Results revealed that curcumin could significantly improve the survival capacity of <i>C. elegans</i> without influencing its growth. Curcumin was observed to significantly decrease the levels of reactive oxygen species (ROS) under extr2023-01-01T00:00:00Z20232026-04-07T13:30:09.532Z2025-02-19T04:30:18.193ZS-EPMC102798903734629910.3389/fphar.2023.1195490