biostudies-literatureJiang FAmerican Heart AssociationNHLBI NIH HHSNational Institutes of HealthNIHUniversity of Rochester Medical CenterNIGMS NIH HHS5831-5846https://www.ebi.ac.uk/biostudies/studies/S-EPMC10287950biostudies-literatureUnknown51(11)Accumulating evidence suggests that posttranscriptional control of gene expression, including RNA splicing, transport, modification, translation and degradation, primarily relies on RNA binding proteins (RBPs). However, the functions of many RBPs remain understudied. Here, we characterized the function of a novel RBP, Proline-Rich Coiled-coil 2B (PRRC2B). Through photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation and sequencing (PAR-CLIP-seq), we identified transcriptome-wide CU- or GA-rich PRRC2B binding sites near the translation initiation codon on a specific cohort of mRNAs in HEK293T cells. These mRNAs, including oncogenes and cell cycle regulators such as CCND2 (cyclin D2), exhibited decreased translation upon PRRC2B knockdown as revealed by polysome-associNucleic acids researchRNA binding protein PRRC2B mediates translation of specific mRNAs and regulates cell cycle progression.PMC10287950R01 HL164584R01 HL147954T32 GM068411848985R01 HL132899Wu JAuguste MYao PJiang FHedaya OMKhor EfalseRNA binding protein PRRC2B mediates translation of specific mRNAs and regulates cell cycle progression.Accumulating evidence suggests that posttranscriptional control of gene expression, including RNA splicing, transport, modification, translation and degradation, primarily relies on RNA binding proteins (RBPs). However, the functions of many RBPs remain understudied. Here, we characterized the function of a novel RBP, Proline-Rich Coiled-coil 2B (PRRC2B). Through photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation and sequencing (PAR-CLIP-seq), we identified transcriptome-wide CU- or GA-rich PRRC2B binding sites near the translation initiation codon on a specific cohort of mRNAs in HEK293T cells. These mRNAs, including oncogenes and cell cycle regulators such as CCND2 (cyclin D2), exhibited decreased translation upon PRRC2B knockdown as revealed by polysome-associ2023-01-01T00:00:00Z2023 Jun2026-04-08T14:38:43.136Z2025-02-19T03:20:06.627ZS-EPMC102879503712563910.1093/nar/gkad322