{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yamada K"],"funding":["Japan Agency for Medical Research and Development","Japan Society for the Promotion of Science"],"pagination":["10243"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10290147"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Polypeptide tags and biotin labelling technologies are widely used for protein analyses in biochemistry and cell biology. However, many peptide tag epitopes contain lysine residues (or amino acids) that are masked after biotinylation. Here, we propose the GATS tag system without a lysine residue and with high sensitivity and low non-specific binding using a rabbit monoclonal antibody against Plasmodium falciparum glycosylphosphatidylinositol (GPI)-anchored micronemal antigen (PfGAMA). From 14 monoclonal clones, an Ra3 clone was selected as it recognized an epitope-TLSVGVQNTF-without a lysine residue; this antibody and epitope tag set was called the GATS tag system. Surface plasmon resonance analysis showed that the tag system had a high affinity of 8.71 × 10<sup>-9</sup> M. GATS tag indica"],"journal":["Scientific reports"],"pubmed_title":["GATS tag system is compatible with biotin labelling methods for protein analysis."],"pmcid":["PMC10290147"],"funding_grant_id":["JP21am0101077","JP16H06579"],"pubmed_authors":["Takashima E","Soga F","Nagaoka H","Sawasaki T","Kishi H","Tokunaga S","Ozawa T","Yamada K"],"additional_accession":[]},"is_claimable":false,"name":"GATS tag system is compatible with biotin labelling methods for protein analysis.","description":"Polypeptide tags and biotin labelling technologies are widely used for protein analyses in biochemistry and cell biology. However, many peptide tag epitopes contain lysine residues (or amino acids) that are masked after biotinylation. Here, we propose the GATS tag system without a lysine residue and with high sensitivity and low non-specific binding using a rabbit monoclonal antibody against Plasmodium falciparum glycosylphosphatidylinositol (GPI)-anchored micronemal antigen (PfGAMA). From 14 monoclonal clones, an Ra3 clone was selected as it recognized an epitope-TLSVGVQNTF-without a lysine residue; this antibody and epitope tag set was called the GATS tag system. Surface plasmon resonance analysis showed that the tag system had a high affinity of 8.71 × 10<sup>-9</sup> M. GATS tag indica","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-04T12:11:12.569Z","creation":"2025-04-04T12:11:12.569Z"},"accession":"S-EPMC10290147","cross_references":{"pubmed":["37353572"],"doi":["10.1038/s41598-023-36858-y"]}}