biostudies-literatureLiu ZBiotechnology and Biological Sciences Research Council1106693https://www.ebi.ac.uk/biostudies/studies/S-EPMC10294229biostudies-literatureUnknown14Patients with occupational medicamentose-like dermatitis due to trichloroethylene often suffer from immune kidney injury. Our previous study reveals that C5b-9-dependent cytosolic Ca²⁺ overload-induced ferroptosis is involved in trichloroethylene sensitized kidney injury. However, how C5b-9 causes cytosolic Ca²⁺ rise and the specific mechanism whereby overloaded Ca²⁺ induces ferroptosis remain unknown. The purpose of our study was to explore the role of IP3R-dependent mitochondrial dysfunction in C5b-9 mediated ferroptosis in trichloroethylene sensitized kidney. Our results showed that IP3R was activated, and mitochondrial membrane potential was decreased in the renal epithelial cells of trichloroethylene-sensitized mice, and these changes were antagonized Frontiers in immunologyIP3R-dependent mitochondrial dysfunction mediates C5b-9-induced ferroptosis in trichloroethylene-caused immune kidney injury.PMC10294229BB/P004695/1Liu ZXie HZhang JZuo XZhang XWu CWang FMa JZhu QfalseIP3R-dependent mitochondrial dysfunction mediates C5b-9-induced ferroptosis in trichloroethylene-caused immune kidney injury.Patients with occupational medicamentose-like dermatitis due to trichloroethylene often suffer from immune kidney injury. Our previous study reveals that C5b-9-dependent cytosolic Ca²⁺ overload-induced ferroptosis is involved in trichloroethylene sensitized kidney injury. However, how C5b-9 causes cytosolic Ca²⁺ rise and the specific mechanism whereby overloaded Ca²⁺ induces ferroptosis remain unknown. The purpose of our study was to explore the role of IP3R-dependent mitochondrial dysfunction in C5b-9 mediated ferroptosis in trichloroethylene sensitized kidney. Our results showed that IP3R was activated, and mitochondrial membrane potential was decreased in the renal epithelial cells of trichloroethylene-sensitized mice, and these changes were antagonized 2023-01-01T00:00:00Z20232025-04-04T12:11:31.034Z2025-04-04T12:11:31.034ZS-EPMC102942293738322410.3389/fimmu.2023.1106693