{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(6)"],"submitter":["Rodel J"],"pubmed_abstract":["The acquisition of hypervirulence-associated genes by carbapenemase-producing <i>Klebsiella pneumoniae</i> is being increasingly observed, and easy-to-use diagnostic tests are needed for the surveillance of the hypervirulent <i>K. pneumoniae</i> (hvKp). In this pilot study, 87 <i>K. pneumoniae</i> isolates from invasive infections collected in 2022 and 2023 were analysed using the LAMP-based eazyplex<sup>®</sup> Superbug CRE and hvKp assays for the simultaneous identification of carbapenemases and virulence genes (<i>rmpA/A2</i>, <i>iuC</i>, <i>iroC</i>, <i>ybt</i>, <i>clb</i>). Nine isolates showed a Kleborate virulence score of 4 or 5 (10.3%). The time for the results of the eazyplex<sup>®</sup> assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation"],"journal":["Antibiotics (Basel, Switzerland)"],"pagination":["959"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10294845"],"repository":["biostudies-literature"],"pubmed_title":["Screening of <i>Klebsiella pneumoniae</i> Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex<sup>®</sup> Superbug CRE and hvKp Assays."],"pmcid":["PMC10294845"],"pubmed_authors":["Fischer MA","Pfister W","Rodel J","Loffler B","Edel B","Stoll S","Pfeifer Y"],"additional_accession":[]},"is_claimable":false,"name":"Screening of <i>Klebsiella pneumoniae</i> Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex<sup>®</sup> Superbug CRE and hvKp Assays.","description":"The acquisition of hypervirulence-associated genes by carbapenemase-producing <i>Klebsiella pneumoniae</i> is being increasingly observed, and easy-to-use diagnostic tests are needed for the surveillance of the hypervirulent <i>K. pneumoniae</i> (hvKp). In this pilot study, 87 <i>K. pneumoniae</i> isolates from invasive infections collected in 2022 and 2023 were analysed using the LAMP-based eazyplex<sup>®</sup> Superbug CRE and hvKp assays for the simultaneous identification of carbapenemases and virulence genes (<i>rmpA/A2</i>, <i>iuC</i>, <i>iroC</i>, <i>ybt</i>, <i>clb</i>). Nine isolates showed a Kleborate virulence score of 4 or 5 (10.3%). The time for the results of the eazyplex<sup>®</sup> assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 May","modification":"2025-04-18T19:17:26.024Z","creation":"2024-12-03T20:33:28.129Z"},"accession":"S-EPMC10294845","cross_references":{"pubmed":["37370278"],"doi":["10.3390/antibiotics12060959"]}}