biostudies-literatureUnknown12(6)Rodel JThe acquisition of hypervirulence-associated genes by carbapenemase-producing <i>Klebsiella pneumoniae</i> is being increasingly observed, and easy-to-use diagnostic tests are needed for the surveillance of the hypervirulent <i>K. pneumoniae</i> (hvKp). In this pilot study, 87 <i>K. pneumoniae</i> isolates from invasive infections collected in 2022 and 2023 were analysed using the LAMP-based eazyplex^® Superbug CRE and hvKp assays for the simultaneous identification of carbapenemases and virulence genes (<i>rmpA/A2</i>, <i>iuC</i>, <i>iroC</i>, <i>ybt</i>, <i>clb</i>). Nine isolates showed a Kleborate virulence score of 4 or 5 (10.3%). The time for the results of the eazyplex^® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparationAntibiotics (Basel, Switzerland)959https://www.ebi.ac.uk/biostudies/studies/S-EPMC10294845biostudies-literatureScreening of <i>Klebsiella pneumoniae</i> Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex^® Superbug CRE and hvKp Assays.PMC10294845Fischer MAPfister WRodel JLoffler BEdel BStoll SPfeifer YfalseScreening of <i>Klebsiella pneumoniae</i> Isolates for Carbapenemase and Hypervirulence-Associated Genes by Combining the Eazyplex^® Superbug CRE and hvKp Assays.The acquisition of hypervirulence-associated genes by carbapenemase-producing <i>Klebsiella pneumoniae</i> is being increasingly observed, and easy-to-use diagnostic tests are needed for the surveillance of the hypervirulent <i>K. pneumoniae</i> (hvKp). In this pilot study, 87 <i>K. pneumoniae</i> isolates from invasive infections collected in 2022 and 2023 were analysed using the LAMP-based eazyplex^® Superbug CRE and hvKp assays for the simultaneous identification of carbapenemases and virulence genes (<i>rmpA/A2</i>, <i>iuC</i>, <i>iroC</i>, <i>ybt</i>, <i>clb</i>). Nine isolates showed a Kleborate virulence score of 4 or 5 (10.3%). The time for the results of the eazyplex^® assays ranged from 6.5 to 13 min, and the total turnaround time, including sample preparation2023-01-01T00:00:00Z2023 May2025-04-18T19:17:26.024Z2024-12-03T20:33:28.129ZS-EPMC102948453737027810.3390/antibiotics12060959