biostudies-literatureCouto-Rodriguez RLNIH HHS1203https://www.ebi.ac.uk/biostudies/studies/S-EPMC10294847biostudies-literatureUnknown12(6)Oxidative stress adaptation strategies are important to cell function and are linked to cardiac, neurodegenerative disease, and cancer. Representatives of the <i>Archaea</i> domain are used as model organisms based on their extreme tolerance to oxidants and close evolutionary relationship with eukaryotes. A study of the halophilic archaeon <i>Haloferax volcanii</i> reveals lysine acetylation to be associated with oxidative stress responses. The strong oxidant hypochlorite: (i) stimulates an increase in lysine acetyltransferase <i>Hv</i>Pat2 to <i>Hv</i>Pat1 abundance ratios and (ii) selects for lysine deacetylase <i>sir2</i> mutants. Here we report the dynamic occupancy of the lysine acetylome of glycerol-grown <i>H. volcanii</i> as it shifts in profile in response to hypochlorite. These fAntioxidants (Basel, Switzerland)Insights into the Lysine Acetylome of the Haloarchaeon <i>Haloferax volcanii</i> during Oxidative Stress by Quantitative SILAC-Based Proteomics.PMC10294847NIH R01 GM57498-20Couto-Rodriguez RLKoh JChen SMaupin-Furlow JAfalseInsights into the Lysine Acetylome of the Haloarchaeon <i>Haloferax volcanii</i> during Oxidative Stress by Quantitative SILAC-Based Proteomics.Oxidative stress adaptation strategies are important to cell function and are linked to cardiac, neurodegenerative disease, and cancer. Representatives of the <i>Archaea</i> domain are used as model organisms based on their extreme tolerance to oxidants and close evolutionary relationship with eukaryotes. A study of the halophilic archaeon <i>Haloferax volcanii</i> reveals lysine acetylation to be associated with oxidative stress responses. The strong oxidant hypochlorite: (i) stimulates an increase in lysine acetyltransferase <i>Hv</i>Pat2 to <i>Hv</i>Pat1 abundance ratios and (ii) selects for lysine deacetylase <i>sir2</i> mutants. Here we report the dynamic occupancy of the lysine acetylome of glycerol-grown <i>H. volcanii</i> as it shifts in profile in response to hypochlorite. These f2023-01-01T00:00:00Z2023 Jun2025-04-22T09:47:18.973Z2025-04-05T23:14:06.206ZS-EPMC102948473737193310.3390/antiox12061203