biostudies-literaturePahlow SSARS-CoV-2DxCarl Zeiss FoundationPhotonics Research GermanyFederal Ministry of Education and Research594https://www.ebi.ac.uk/biostudies/studies/S-EPMC10296004biostudies-literatureUnknown13(6)We introduce a magnetic bead-based sample preparation scheme for enabling the Raman spectroscopic differentiation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-positive and -negative samples. The beads were functionalized with the angiotensin-converting enzyme 2 (ACE2) receptor protein, which is used as a recognition element to selectively enrich SARS-CoV-2 on the surface of the magnetic beads. The subsequent Raman measurements directly enable discriminating SARS-CoV-2-positive and -negative samples. The proposed approach is also applicable for other virus species when the specific recognition element is exchanged. A series of Raman spectra were measured on three types of samples, namely SARS-CoV-2, Influenza A H1N1 virus and a negative control. For each sample type,BiosensorsSimple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy.PMC1029600413N15708, 13N1546613N1570813N15745P2018-01-00413N15466Weber KRyabchykov ORichard-Lacroix MEhricht RHniopek JLoffler BDeinhardt-Emmer SPahlow SKose-Vogel NHornung FMayerhofer TGPopp JBocklitz TfalseSimple, Fast and Convenient Magnetic Bead-Based Sample Preparation for Detecting Viruses via Raman-Spectroscopy.We introduce a magnetic bead-based sample preparation scheme for enabling the Raman spectroscopic differentiation of severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2)-positive and -negative samples. The beads were functionalized with the angiotensin-converting enzyme 2 (ACE2) receptor protein, which is used as a recognition element to selectively enrich SARS-CoV-2 on the surface of the magnetic beads. The subsequent Raman measurements directly enable discriminating SARS-CoV-2-positive and -negative samples. The proposed approach is also applicable for other virus species when the specific recognition element is exchanged. A series of Raman spectra were measured on three types of samples, namely SARS-CoV-2, Influenza A H1N1 virus and a negative control. For each sample type,2023-01-01T00:00:00Z2023 May2026-04-08T12:39:48.985Z2025-04-07T10:22:21.097ZS-EPMC102960043736695910.3390/bios13060594