biostudies-literatureChiantore MVIstituto Superiore di SanitàSapienza University of Rome1719https://www.ebi.ac.uk/biostudies/studies/S-EPMC10296342biostudies-literatureUnknown11(6)Actinic keratosis (AK) is a carcinoma in situ precursor of cutaneous squamous cell carcinoma (cSCC), the second most common cancer affecting the Caucasian population. AK is frequently present in the sun-exposed skin of the elderly population, UV radiation being the main cause of this cancer, and other risk factors contributing to AK incidence. The dysregulation of microRNAs (miRNAs) observed in different cancers leads to an improper expression of miRNA targets involved in several cellular pathways. The TaqMan Array Human MicroRNA Card assay for miRNA expression profiling was performed in pooled AK compared to healthy skin scraping samples from the same patients. Forty-three miRNAs were modulated in the AK samples. The expression of miR-19b (<i>p</i> < 0.05), -31, -34a (<i>p</i> < 0.001), -BiomedicinesMicroRNAs Differentially Expressed in Actinic Keratosis and Healthy Skin Scrapings.PMC10296342not applicableBando di Ateneo Sapienza-2020", Grant no. RM120172B8B7B997Iuliano MLuzi FFiorucci GMangino GRomeo GGrimaldi LAccardi LChiantore MVMongiovi RMDi Bonito PfalseMicroRNAs Differentially Expressed in Actinic Keratosis and Healthy Skin Scrapings.Actinic keratosis (AK) is a carcinoma in situ precursor of cutaneous squamous cell carcinoma (cSCC), the second most common cancer affecting the Caucasian population. AK is frequently present in the sun-exposed skin of the elderly population, UV radiation being the main cause of this cancer, and other risk factors contributing to AK incidence. The dysregulation of microRNAs (miRNAs) observed in different cancers leads to an improper expression of miRNA targets involved in several cellular pathways. The TaqMan Array Human MicroRNA Card assay for miRNA expression profiling was performed in pooled AK compared to healthy skin scraping samples from the same patients. Forty-three miRNAs were modulated in the AK samples. The expression of miR-19b (<i>p</i> < 0.05), -31, -34a (<i>p</i> < 0.001), -2023-01-01T00:00:00Z2023 Jun2025-04-19T09:00:27.314Z2025-04-19T09:00:27.314ZS-EPMC102963423737181410.3390/biomedicines11061719