{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(12)"],"submitter":["Aouimeur I"],"pubmed_abstract":["Corneal endothelial diseases are the leading cause of corneal transplantation. The global shortage of donor corneas has resulted in the investigation of alternative methods, such as cell therapy and tissue-engineered endothelial keratoplasty (TEEK), using primary cultures of human corneal endothelial cells (hCECs). The main challenge is optimizing the hCEC culture process to increase the endothelial cell density (ECD) and overall yield while preventing endothelial-mesenchymal transition (EndMT). Fetal bovine serum (FBS) is necessary for hCEC expansion but contains TGF-βs, which have been shown to be detrimental to hCECs. Therefore, we investigated various TGF-β signaling pathways using inhibitors to improve hCEC culture. Initially, we confirmed that TGF-β1, 2, and 3 induced EndMT on conflu"],"journal":["Cells"],"pagination":["1624"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10297110"],"repository":["biostudies-literature"],"pubmed_title":["Investigating the Role of TGF-β Signaling Pathways in Human Corneal Endothelial Cell Primary Culture."],"pmcid":["PMC10297110"],"pubmed_authors":["Aouimeur I","Forest F","Thuret G","Coulomb L","He Z","Maurin C","Thomas J","Sagnial T","Ninotta S","Forestier P","Perrache C","Gain P"],"additional_accession":[]},"is_claimable":false,"name":"Investigating the Role of TGF-β Signaling Pathways in Human Corneal Endothelial Cell Primary Culture.","description":"Corneal endothelial diseases are the leading cause of corneal transplantation. The global shortage of donor corneas has resulted in the investigation of alternative methods, such as cell therapy and tissue-engineered endothelial keratoplasty (TEEK), using primary cultures of human corneal endothelial cells (hCECs). The main challenge is optimizing the hCEC culture process to increase the endothelial cell density (ECD) and overall yield while preventing endothelial-mesenchymal transition (EndMT). Fetal bovine serum (FBS) is necessary for hCEC expansion but contains TGF-βs, which have been shown to be detrimental to hCECs. Therefore, we investigated various TGF-β signaling pathways using inhibitors to improve hCEC culture. Initially, we confirmed that TGF-β1, 2, and 3 induced EndMT on conflu","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2026-06-22T03:14:40.356Z","creation":"2025-04-04T12:11:40.992Z"},"accession":"S-EPMC10297110","cross_references":{"pubmed":["37371094"],"doi":["10.3390/cells12121624"]}}