biostudies-literatureYin XChina Agriculture Research SystemSchool-level Research Projects of the Yancheng Institute of TechnologyNatural Science Foundation of Jiangsu ProvinceNational Broiler Industrial and Technology System1213https://www.ebi.ac.uk/biostudies/studies/S-EPMC10298623biostudies-literatureUnknown14(6)Skeletal muscle growth and development from embryo to adult consists of a series of carefully regulated changes in gene expression. This study aimed to identify candidate genes involved in Haiyang Yellow Chickens' growth and to understand the regulatory role of the key gene <i>ALOX5</i> (arachidonate 5-lipoxygenase) in myoblast proliferation and differentiation. In order to search the key candidate genes in the process of muscle growth and development, RNA sequencing was used to compare the transcriptomes of chicken muscle tissues at four developmental stages and to analyze the effects of <i>ALOX5</i> gene interference and overexpression on myoblast proliferation and differentiation at the cellular level. The results showed that 5743 differentially expressed genes (DEGs) (|fold change| ≥ 2GenesTranscriptome Analysis of Leg Muscles and the Effects of <i>ALOX5</i> on Proliferation and Differentiation of Myoblasts in Haiyang Yellow Chickens.PMC10298623Xjr20210201BK20210944nycytx-42-G1-05CARS-41Yin XFang WYuan MSun HWang JfalseTranscriptome Analysis of Leg Muscles and the Effects of <i>ALOX5</i> on Proliferation and Differentiation of Myoblasts in Haiyang Yellow Chickens.Skeletal muscle growth and development from embryo to adult consists of a series of carefully regulated changes in gene expression. This study aimed to identify candidate genes involved in Haiyang Yellow Chickens' growth and to understand the regulatory role of the key gene <i>ALOX5</i> (arachidonate 5-lipoxygenase) in myoblast proliferation and differentiation. In order to search the key candidate genes in the process of muscle growth and development, RNA sequencing was used to compare the transcriptomes of chicken muscle tissues at four developmental stages and to analyze the effects of <i>ALOX5</i> gene interference and overexpression on myoblast proliferation and differentiation at the cellular level. The results showed that 5743 differentially expressed genes (DEGs) (|fold change| ≥ 22023-01-01T00:00:00Z2023 Jun2026-04-08T12:41:46.976Z2025-04-07T10:22:18.204ZS-EPMC102986233737239310.3390/genes14061213