biostudies-literatureUnknown14(6)Bogale M<i>Trichopoda pennipes</i> is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, <i>Nezara viridula</i>, and leaf-footed bug, <i>Leptoglossus phyllopus</i>. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of <i>T. pennipes</i> were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected <i>N. viridula</i> and <i>L. phyllopus</i>. High-quality de novo draft genomes of <i>T. pennipes</i> were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was aGenes1172https://www.ebi.ac.uk/biostudies/studies/S-EPMC10298747biostudies-literatureFirst Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of <i>Trichopoda pennipes</i>, a Parasitoid of <i>Nezara viridula</i>.PMC10298747Bogale MDiaz AYun MRooney LAuletta AHacker JLee FBossert KMattia ADiGennaro PSeslija MChau QLeppla NBarreto PHadaegh LOverton GReis VSalichs OBishop GChan LHibshman TMishra SVemugunta VBremer KStylianou NRoeder EBustamante DNguyen BRush MCordo JLastra KRhodes DStacey KfalseFirst Description of the Nuclear and Mitochondrial Genomes and Associated Host Preference of <i>Trichopoda pennipes</i>, a Parasitoid of <i>Nezara viridula</i>.<i>Trichopoda pennipes</i> is a tachinid parasitoid of several significant heteropteran agricultural pests, including the southern green stink bug, <i>Nezara viridula</i>, and leaf-footed bug, <i>Leptoglossus phyllopus</i>. To be used successfully as a biological control agent, the fly must selectively parasitize the target host species. Differences in the host preference of <i>T. pennipes</i> were assessed by assembling the nuclear and mitochondrial genomes of 38 flies reared from field-collected <i>N. viridula</i> and <i>L. phyllopus</i>. High-quality de novo draft genomes of <i>T. pennipes</i> were assembled using long-read sequencing. The assembly totaled 672 MB distributed among 561 contigs, having an N50 of 11.9 MB and a GC of 31.7%, with the longest contig at 28 MB. The genome was a2023-01-01T00:00:00Z2023 May2026-06-23T03:22:49.033Z2025-04-07T10:22:07.568ZS-EPMC102987473737235210.3390/genes14061172