{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Saleh DO"],"funding":["Deutscher Akademischer Austauschdienst","European Research Council","Volkswagen Foundation","Ministry of Scientific Research, Egypt"],"pagination":["e1011451"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10298799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["19(6)"],"pubmed_abstract":["Both the bacterial flagellum and the evolutionary related injectisome encoded on the Salmonella pathogenicity island 1 (SPI-1) play crucial roles during the infection cycle of Salmonella species. The interplay of both is highlighted by the complex cross-regulation that includes transcriptional control of the flagellar master regulatory operon flhDC by HilD, the master regulator of SPI-1 gene expression. Contrary to the HilD-dependent activation of flagellar gene expression, we report here that activation of HilD resulted in a dramatic loss of motility, which was dependent on the presence of SPI-1. Single cell analyses revealed that HilD-activation triggers a SPI-1-dependent induction of the stringent response and a substantial decrease in proton motive force (PMF), while flagellation remai"],"journal":["PLoS pathogens"],"pubmed_title":["SPI-1 virulence gene expression modulates motility of Salmonella Typhimurium in a proton motive force- and adhesins-dependent manner."],"pmcid":["PMC10298799"],"funding_grant_id":["864971","91705821"],"pubmed_authors":["Saleh DO","Erhardt M","Klotzsch E","Weber W","Giralt-Zuniga M","Durairaj AC","Kaganovitch E","Strowig T","Horstmann JA"],"additional_accession":[]},"is_claimable":false,"name":"SPI-1 virulence gene expression modulates motility of Salmonella Typhimurium in a proton motive force- and adhesins-dependent manner.","description":"Both the bacterial flagellum and the evolutionary related injectisome encoded on the Salmonella pathogenicity island 1 (SPI-1) play crucial roles during the infection cycle of Salmonella species. The interplay of both is highlighted by the complex cross-regulation that includes transcriptional control of the flagellar master regulatory operon flhDC by HilD, the master regulator of SPI-1 gene expression. Contrary to the HilD-dependent activation of flagellar gene expression, we report here that activation of HilD resulted in a dramatic loss of motility, which was dependent on the presence of SPI-1. Single cell analyses revealed that HilD-activation triggers a SPI-1-dependent induction of the stringent response and a substantial decrease in proton motive force (PMF), while flagellation remai","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jun","modification":"2025-04-26T04:54:45.55Z","creation":"2025-04-06T11:14:21.607Z"},"accession":"S-EPMC10298799","cross_references":{"pubmed":["37315106"],"doi":["10.1371/journal.ppat.1011451"]}}