biostudies-literatureKim MKNational Research Foundation of Korea10182https://www.ebi.ac.uk/biostudies/studies/S-EPMC10298908biostudies-literatureUnknown24(12)Novel genetic and epigenetic factors involved in the development and prognosis of idiopathic pulmonary fibrosis (IPF) have been identified. We previously observed that erythrocyte membrane protein band 4.1-like 3 (<i>EPB41L3</i>) increased in the lung fibroblasts of IPF patients. Thus, we investigated the role of <i>EPB41L3</i> in IPF by comparing the EPB41L3 mRNA and protein expression of lung fibroblast between patients with IPF and controls. We also investigated the regulation of epithelial-mesenchymal transition (EMT) in an epithelial cell line (A549) and fibroblast-to-myofibroblast transition (FMT) in a fibroblast cell line (MRC5) by overexpressing and silencing <i>EPB41L3</i>. EPB41L3 mRNA and protein levels, as measured using RT-PCR, real-time PCR, and Western blot, were significantInternational journal of molecular sciencesThe Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis.PMC10298908NRF-2022R1A2C1010172Lee JULee SJPark CSKim MKChang HSPark JSfalseThe Role of Erythrocyte Membrane Protein Band 4.1-like 3 in Idiopathic Pulmonary Fibrosis.Novel genetic and epigenetic factors involved in the development and prognosis of idiopathic pulmonary fibrosis (IPF) have been identified. We previously observed that erythrocyte membrane protein band 4.1-like 3 (<i>EPB41L3</i>) increased in the lung fibroblasts of IPF patients. Thus, we investigated the role of <i>EPB41L3</i> in IPF by comparing the EPB41L3 mRNA and protein expression of lung fibroblast between patients with IPF and controls. We also investigated the regulation of epithelial-mesenchymal transition (EMT) in an epithelial cell line (A549) and fibroblast-to-myofibroblast transition (FMT) in a fibroblast cell line (MRC5) by overexpressing and silencing <i>EPB41L3</i>. EPB41L3 mRNA and protein levels, as measured using RT-PCR, real-time PCR, and Western blot, were significant2023-01-01T00:00:00Z2023 Jun2025-04-18T17:26:22.733Z2025-04-07T04:57:08.2ZS-EPMC102989083737333010.3390/ijms241210182