biostudies-literatureZhang WNational Natural Science Foundation of ChinaPost-graduate’s Innovation Fund Project of Hebei ProvincePost-graduate's Innovation Fund Project of Hebei Province517https://www.ebi.ac.uk/biostudies/studies/S-EPMC10299072biostudies-literatureUnknown14(6)Salticidae is the most species-rich family of spiders with diverse morphology, ecology and behavior. However, the characteristics of the mitogenomes within this group are poorly understood with relatively few well-characterized complete mitochondrial genomes. In this study, we provide completely annotated mitogenomes for <i>Corythalia opima</i> and <i>Parabathippus shelfordi</i>, which represent the first complete mitogenomes of the tribe Euophryini of Salticidae. The features and characteristics of the mitochondrial genomes are elucidated for Salticidae by thoroughly comparing the known well-characterized mitogenomes. The gene rearrangement between <i>trnL2</i> and <i>trnN</i> was found in two jumping spider species, <i>Corythalia opima</i> and <i>Heliophanus lineiventris</i> Simon, 1868. Additionally, the rearrangement of <i>nad1</i> to between <i>trnE</i> and <i>trnF</i> found in <i>Asemonea sichuanensis</i> Song & Chai, 1992 is the first protein-coding gene rearrangement in Salticidae, which may have an important phylogenetic implication for the family. Tandem repeats of various copy numbers and lengths were discovered in three jumping spider species. The codon usage analyses showed that the evolution of codon usage bias in salticid mitogenomes was affected by both selection and mutational pressure, but selection may have played a more important role. The phylogenetic analyses provided insight into the taxonomy of <i>Colopsus longipalpis</i> (Żabka, 1985). The data presented in this study will improve our understanding of the evolution of mitochondrial genomes within Salticidae.InsectsComparative Mitogenomics of Jumping Spiders with First Complete Mitochondrial Genomes of Euophryini (Araneae: Salticidae).PMC1029907232070422CXZZSS2023009Zhang WZhang FDing YZhang JLin LfalseComparative Mitogenomics of Jumping Spiders with First Complete Mitochondrial Genomes of Euophryini (Araneae: Salticidae).Salticidae is the most species-rich family of spiders with diverse morphology, ecology and behavior. However, the characteristics of the mitogenomes within this group are poorly understood with relatively few well-characterized complete mitochondrial genomes. In this study, we provide completely annotated mitogenomes for <i>Corythalia opima</i> and <i>Parabathippus shelfordi</i>, which represent the first complete mitogenomes of the tribe Euophryini of Salticidae. The features and characteristics of the mitochondrial genomes are elucidated for Salticidae by thoroughly comparing the known well-characterized mitogenomes. The gene rearrangement between <i>trnL2</i> and <i>trnN</i> was found in two jumping spider species, <i>Corythalia opima</i> and <i>Heliophanus lineiventris</i> Simon, 1868. Additionally, the rearrangement of <i>nad1</i> to between <i>trnE</i> and <i>trnF</i> found in <i>Asemonea sichuanensis</i> Song & Chai, 1992 is the first protein-coding gene rearrangement in Salticidae, which may have an important phylogenetic implication for the family. Tandem repeats of various copy numbers and lengths were discovered in three jumping spider species. The codon usage analyses showed that the evolution of codon usage bias in salticid mitogenomes was affected by both selection and mutational pressure, but selection may have played a more important role. The phylogenetic analyses provided insight into the taxonomy of <i>Colopsus longipalpis</i> (Żabka, 1985). The data presented in this study will improve our understanding of the evolution of mitochondrial genomes within Salticidae.2023-01-01T00:00:00Z2023 Jun2026-04-08T12:37:37.166Z2025-04-07T10:19:29.62ZS-EPMC102990723736733310.3390/insects14060517