biostudies-literatureUnknown24(12)Kanda YCrops experience herbivory by arthropods and microbial infections. In the interaction between plants and chewing herbivores, lepidopteran larval oral secretions (OS) and plant-derived damage-associated molecular patterns (DAMPs) trigger plant defense responses. However, the mechanisms underlying anti-herbivore defense, especially in monocots, have not been elucidated. The receptor-like cytoplasmic kinase Broad-Spectrum Resistance 1 (BSR1) of <i>Oryza sativa</i> L. (rice) mediates cytoplasmic defense signaling in response to microbial pathogens and enhances disease resistance when overexpressed. Here, we investigated whether BSR1 contributes to anti-herbivore defense responses. <i>BSR1</i> knockout suppressed rice responses triggered by OS from the chewing herbivore <i>Mythimna loreyi</i> Duponchel (Lepidoptera: Noctuidae) and peptidic DAMPs OsPeps, including the activation of genes required for biosynthesis of diterpenoid phytoalexins (DPs). <i>BSR1</i>-overexpressing rice plants exhibited hyperactivation of DP accumulation and ethylene signaling after treatment with simulated herbivory and acquired enhanced resistance to larval feeding. As the biological significance of herbivory-induced accumulation of rice DPs remains unexplained, their physiological activities in <i>M. loreyi</i> were analyzed. The addition of momilactone B, a rice DP, to the artificial diet suppressed the growth of <i>M. loreyi</i> larvae. Altogether, this study revealed that BSR1 and herbivory-induced rice DPs are involved in the defense against chewing insects, in addition to pathogens.International journal of molecular sciences10395https://www.ebi.ac.uk/biostudies/studies/S-EPMC10299516biostudies-literatureBSR1, a Rice Receptor-like Cytoplasmic Kinase, Positively Regulates Defense Responses to Herbivory.PMC10299516Mori MMujiono KMaeda SOkada KGalis IKanda YShinya TTomita KKamakura THojo YfalseBSR1, a Rice Receptor-like Cytoplasmic Kinase, Positively Regulates Defense Responses to Herbivory.Crops experience herbivory by arthropods and microbial infections. In the interaction between plants and chewing herbivores, lepidopteran larval oral secretions (OS) and plant-derived damage-associated molecular patterns (DAMPs) trigger plant defense responses. However, the mechanisms underlying anti-herbivore defense, especially in monocots, have not been elucidated. The receptor-like cytoplasmic kinase Broad-Spectrum Resistance 1 (BSR1) of <i>Oryza sativa</i> L. (rice) mediates cytoplasmic defense signaling in response to microbial pathogens and enhances disease resistance when overexpressed. Here, we investigated whether BSR1 contributes to anti-herbivore defense responses. <i>BSR1</i> knockout suppressed rice responses triggered by OS from the chewing herbivore <i>Mythimna loreyi</i> Duponchel (Lepidoptera: Noctuidae) and peptidic DAMPs OsPeps, including the activation of genes required for biosynthesis of diterpenoid phytoalexins (DPs). <i>BSR1</i>-overexpressing rice plants exhibited hyperactivation of DP accumulation and ethylene signaling after treatment with simulated herbivory and acquired enhanced resistance to larval feeding. As the biological significance of herbivory-induced accumulation of rice DPs remains unexplained, their physiological activities in <i>M. loreyi</i> were analyzed. The addition of momilactone B, a rice DP, to the artificial diet suppressed the growth of <i>M. loreyi</i> larvae. Altogether, this study revealed that BSR1 and herbivory-induced rice DPs are involved in the defense against chewing insects, in addition to pathogens.2023-01-01T00:00:00Z2023 Jun2025-04-04T12:11:41.259Z2025-04-04T12:11:41.259ZS-EPMC102995163737354610.3390/ijms241210395