{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Weng SC"],"funding":["NIAID NIH HHS","NIGMS NIH HHS"],"pubmed_abstract":["Only female mosquitoes consume blood and transmit deadly human pathogens. Therefore, it is critical to remove females before conducting releases for genetic biocontrol interventions. Here we describe a robust sex-sorting approach termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) that exploits sex-specific alternative splicing of an innocuous reporter to ensure exclusive dominant male-specific expression. Using SEPARATOR, we demonstrate reliable sex selection from larval and pupal stages in <i>Aedes aegypti</i>, and use a Complex Object Parametric Analyzer and Sorter (COPAS<sup>®</sup>) to demonstrate scalable high-throughput sex-selection of first instar larvae. Additionally, we use this approach to sequence the transcriptomes of ear"],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.06.16.545348"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10312783"],"repository":["biostudies-literature"],"pubmed_title":["Efficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in <i>Aedes aegypti</i>."],"pmcid":["PMC10312783"],"funding_grant_id":["R01 AI175152","R01 GM132825","R01 AI151004","DP2 AI152071","R01 AI148300"],"pubmed_authors":["Antoshechkin I","Akbari OS","Weng SC","Marois E"],"additional_accession":[]},"is_claimable":false,"name":"Efficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in <i>Aedes aegypti</i>.","description":"Only female mosquitoes consume blood and transmit deadly human pathogens. Therefore, it is critical to remove females before conducting releases for genetic biocontrol interventions. Here we describe a robust sex-sorting approach termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) that exploits sex-specific alternative splicing of an innocuous reporter to ensure exclusive dominant male-specific expression. Using SEPARATOR, we demonstrate reliable sex selection from larval and pupal stages in <i>Aedes aegypti</i>, and use a Complex Object Parametric Analyzer and Sorter (COPAS<sup>®</sup>) to demonstrate scalable high-throughput sex-selection of first instar larvae. Additionally, we use this approach to sequence the transcriptomes of ear","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2026-04-08T13:47:09.711Z","creation":"2024-11-13T10:23:34.861Z"},"accession":"S-EPMC10312783","cross_references":{"pubmed":["37398094"],"doi":["10.1101/2023.06.16.545348"]}}