biostudies-literatureUnknownWeng SCNIAID NIH HHSNIGMS NIH HHSOnly female mosquitoes consume blood and transmit deadly human pathogens. Therefore, it is critical to remove females before conducting releases for genetic biocontrol interventions. Here we describe a robust sex-sorting approach termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) that exploits sex-specific alternative splicing of an innocuous reporter to ensure exclusive dominant male-specific expression. Using SEPARATOR, we demonstrate reliable sex selection from larval and pupal stages in <i>Aedes aegypti</i>, and use a Complex Object Parametric Analyzer and Sorter (COPAS^®) to demonstrate scalable high-throughput sex-selection of first instar larvae. Additionally, we use this approach to sequence the transcriptomes of earbioRxiv : the preprint server for biology2023.06.16.545348https://www.ebi.ac.uk/biostudies/studies/S-EPMC10312783biostudies-literatureEfficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in <i>Aedes aegypti</i>.PMC10312783R01 AI175152R01 GM132825R01 AI151004DP2 AI152071R01 AI148300Antoshechkin IAkbari OSWeng SCMarois EfalseEfficient Sex Separation by Exploiting Differential Alternative Splicing of a Dominant Marker in <i>Aedes aegypti</i>.Only female mosquitoes consume blood and transmit deadly human pathogens. Therefore, it is critical to remove females before conducting releases for genetic biocontrol interventions. Here we describe a robust sex-sorting approach termed SEPARATOR (Sexing Element Produced by Alternative RNA-splicing of A Transgenic Observable Reporter) that exploits sex-specific alternative splicing of an innocuous reporter to ensure exclusive dominant male-specific expression. Using SEPARATOR, we demonstrate reliable sex selection from larval and pupal stages in <i>Aedes aegypti</i>, and use a Complex Object Parametric Analyzer and Sorter (COPAS^®) to demonstrate scalable high-throughput sex-selection of first instar larvae. Additionally, we use this approach to sequence the transcriptomes of ear2023-01-01T00:00:00Z2023 Jul2026-04-08T13:47:09.711Z2024-11-13T10:23:34.861ZS-EPMC103127833739809410.1101/2023.06.16.545348