{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Bellchambers HM"],"funding":["NCATS NIH HHS","NHLBI NIH HHS","U.S. Department of Health & Human Services | National Institutes of Health"],"pagination":["10688"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10314903"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The establishment of left-right patterning in mice occurs at a transient structure called the embryonic node or left-right organizer (LRO). Previous analysis of the LRO has proven challenging due to the small cell number and transient nature of this structure. Here, we seek to overcome these difficulties to define the transcriptome of the LRO. Specifically, we used single cell RNA sequencing of 0-1 somite embryos to identify LRO enriched genes which were compared to bulk RNA sequencing of LRO cells isolated by fluorescent activated cell sorting. Gene ontology analysis indicated an enrichment of genes associated with cilia and laterality terms. Furthermore, comparison to previously identified LRO genes identified 127 novel LRO genes, including Ttll3, Syne1 and Sparcl1, for which the express"],"journal":["Scientific reports"],"pubmed_title":["Single cell RNA analysis of the left-right organizer transcriptome reveals potential novel heterotaxy genes."],"pmcid":["PMC10314903"],"funding_grant_id":["RO1 HL088639","P01 HL134599","R01 HL088639","P01 HL 134599","UL1 TR002529"],"pubmed_authors":["Liu Y","Ware SM","Bellchambers HM","Padua MB","Gao H","Phatak AR","Nenni MJ"],"additional_accession":[]},"is_claimable":false,"name":"Single cell RNA analysis of the left-right organizer transcriptome reveals potential novel heterotaxy genes.","description":"The establishment of left-right patterning in mice occurs at a transient structure called the embryonic node or left-right organizer (LRO). Previous analysis of the LRO has proven challenging due to the small cell number and transient nature of this structure. Here, we seek to overcome these difficulties to define the transcriptome of the LRO. Specifically, we used single cell RNA sequencing of 0-1 somite embryos to identify LRO enriched genes which were compared to bulk RNA sequencing of LRO cells isolated by fluorescent activated cell sorting. Gene ontology analysis indicated an enrichment of genes associated with cilia and laterality terms. Furthermore, comparison to previously identified LRO genes identified 127 novel LRO genes, including Ttll3, Syne1 and Sparcl1, for which the express","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-07-10T03:08:20.536Z","creation":"2025-04-04T14:08:26.641Z"},"accession":"S-EPMC10314903","cross_references":{"pubmed":["37393374"],"doi":["10.1038/s41598-023-36862-2"]}}