biostudies-literatureMahjour BACS MEDI Predoctoral FellowshipNational Science Foundation3924https://www.ebi.ac.uk/biostudies/studies/S-EPMC10318092biostudies-literatureUnknown14(1)High-throughput experimentation (HTE) is an increasingly important tool in reaction discovery. While the hardware for running HTE in the chemical laboratory has evolved significantly in recent years, there remains a need for software solutions to navigate data-rich experiments. Here we have developed phactor™, a software that facilitates the performance and analysis of HTE in a chemical laboratory. phactor™ allows experimentalists to rapidly design arrays of chemical reactions or direct-to-biology experiments in 24, 96, 384, or 1,536 wellplates. Users can access online reagent data, such as a chemical inventory, to virtually populate wells with experiments and produce instructions to perform the reaction array manually, or with the assistance of a liquid handling robot. After completion ofNature communicationsRapid planning and analysis of high-throughput experiment arrays for reaction discovery.PMC10318092CHE-2236215Mohamed OGDouthwaite JLCernak TZhao RZhang ZLin YTripathi AMahjour BShen YMcGrath AZhang RfalseRapid planning and analysis of high-throughput experiment arrays for reaction discovery.High-throughput experimentation (HTE) is an increasingly important tool in reaction discovery. While the hardware for running HTE in the chemical laboratory has evolved significantly in recent years, there remains a need for software solutions to navigate data-rich experiments. Here we have developed phactor™, a software that facilitates the performance and analysis of HTE in a chemical laboratory. phactor™ allows experimentalists to rapidly design arrays of chemical reactions or direct-to-biology experiments in 24, 96, 384, or 1,536 wellplates. Users can access online reagent data, such as a chemical inventory, to virtually populate wells with experiments and produce instructions to perform the reaction array manually, or with the assistance of a liquid handling robot. After completion of2023-01-01T00:00:00Z2023 Jul2025-04-18T17:51:00.306Z2025-04-07T05:25:05.267ZS-EPMC103180923740046910.1038/s41467-023-39531-0