{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Luchsinger C"],"funding":["NIAID NIH HHS","NIH"],"pagination":["10974"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10325960"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["The early events of HIV-1 infection involve the transport of the viral core into the nucleus. This event triggers the translocation of CPSF6 from paraspeckles into nuclear speckles forming puncta-like structures. Our investigations revealed that neither HIV-1 integration nor reverse transcription is required for the formation of puncta-like structures. Moreover, HIV-1 viruses without viral genome are competent for the induction of CPSF6 puncta-like structures. In agreement with the notion that HIV-1 induced CPSF6 puncta-like structures are biomolecular condensates, we showed that osmotic stress and 1,6-hexanediol induced the disassembly of CPSF6 condensates. Interestingly, replacing the osmotic stress by isotonic media re-assemble CPSF6 condensates in the cytoplasm of the cell. To test whe"],"journal":["Scientific reports"],"pubmed_title":["Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection."],"pmcid":["PMC10325960"],"funding_grant_id":["AI087390","R01 AI087390"],"pubmed_authors":["Luchsinger C","Lee K","Diaz-Griffero F","Mardones GA","KewalRamani VN"],"additional_accession":[]},"is_claimable":false,"name":"Formation of nuclear CPSF6/CPSF5 biomolecular condensates upon HIV-1 entry into the nucleus is important for productive infection.","description":"The early events of HIV-1 infection involve the transport of the viral core into the nucleus. This event triggers the translocation of CPSF6 from paraspeckles into nuclear speckles forming puncta-like structures. Our investigations revealed that neither HIV-1 integration nor reverse transcription is required for the formation of puncta-like structures. Moreover, HIV-1 viruses without viral genome are competent for the induction of CPSF6 puncta-like structures. In agreement with the notion that HIV-1 induced CPSF6 puncta-like structures are biomolecular condensates, we showed that osmotic stress and 1,6-hexanediol induced the disassembly of CPSF6 condensates. Interestingly, replacing the osmotic stress by isotonic media re-assemble CPSF6 condensates in the cytoplasm of the cell. To test whe","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2026-07-15T00:29:44.593Z","creation":"2026-06-27T03:05:45.227Z"},"accession":"S-EPMC10325960","cross_references":{"pubmed":["37414787"],"doi":["10.1038/s41598-023-37364-x"]}}