biostudies-literatureUnknown12(6)Alatas FS<h4>Background</h4>Hypoganglionosis resembles Hirschsprung's disease as in both diseases, patients may present with severe constipation or pseudo-obstruction. To date, diagnosis of hypoganglionosis is still difficult to be established due to lack of international consensus regarding diagnostic criteria. This study aims to evaluate the use of immunohistochemistry to provide objective support for our initial subjective impression of hypoganglionosis as well as to describe the morphological features of this study.<h4>Methods</h4>This is a cross-sectional study. Three resected intestinal samples from patients with hypoganglionosis at Kyushu University Hospital, Fukuoka, Japan were included in this study. One healthy intestinal sample was used as control. All specimens were immunohistochemicallTranslational pediatrics1161-1169https://www.ebi.ac.uk/biostudies/studies/S-EPMC10326751biostudies-literatureDiagnostic challenges of hypoganglionosis based on immunohistochemical method.PMC10326751Alatas FSKadim MMasumoto KPudjiadi AHTajiri TNagata KTaguchi TfalseDiagnostic challenges of hypoganglionosis based on immunohistochemical method.<h4>Background</h4>Hypoganglionosis resembles Hirschsprung's disease as in both diseases, patients may present with severe constipation or pseudo-obstruction. To date, diagnosis of hypoganglionosis is still difficult to be established due to lack of international consensus regarding diagnostic criteria. This study aims to evaluate the use of immunohistochemistry to provide objective support for our initial subjective impression of hypoganglionosis as well as to describe the morphological features of this study.<h4>Methods</h4>This is a cross-sectional study. Three resected intestinal samples from patients with hypoganglionosis at Kyushu University Hospital, Fukuoka, Japan were included in this study. One healthy intestinal sample was used as control. All specimens were immunohistochemicall2023-01-01T00:00:00Z2023 Jun2025-04-20T03:46:11.497Z2025-04-20T03:46:11.497ZS-EPMC103267513742705910.21037/tp-22-592