{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kohler TN"],"funding":["European Research Council","Medical Research Council","Wellcome Trust","Biotechnology and Biological Sciences Research Council"],"pagination":["4022"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10329048"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs will provide insight into mechanisms underlying mammalian pre-implantation development. Here, we explore this type of regulation by exerting microenvironmental control over mouse embryonic stem cells. Microfluidic encapsulation of mouse embryonic stem cells in agarose microgels stabilizes the naive pluripotency network and specifically induces expression of Plakoglobin (Jup), a vertebrate homolog of β-catenin. Overexpression of Plakoglobin is sufficient to fully re-establish the naive pluripotency gene regulatory network under metastable pluripotency conditions, as confirmed by single-cell transcriptome profiling. Fi"],"journal":["Nature communications"],"pubmed_title":["Plakoglobin is a mechanoresponsive regulator of naive pluripotency."],"pmcid":["PMC10329048"],"funding_grant_id":["695669","MC_PC_17230","WT108438/C/15/Z","1795140"],"pubmed_authors":["Herger M","Slatery EM","Kohler TN","Nichols J","De Jonghe J","Ellermann AL","Franze K","Winkel A","Weberling A","Munger C","Hollfelder F","Boroviak TE","Yanagida A","Fischer K","Bergmann S","Mulas C","Ross C","Chalut K"],"additional_accession":[]},"is_claimable":false,"name":"Plakoglobin is a mechanoresponsive regulator of naive pluripotency.","description":"Biomechanical cues are instrumental in guiding embryonic development and cell differentiation. Understanding how these physical stimuli translate into transcriptional programs will provide insight into mechanisms underlying mammalian pre-implantation development. Here, we explore this type of regulation by exerting microenvironmental control over mouse embryonic stem cells. Microfluidic encapsulation of mouse embryonic stem cells in agarose microgels stabilizes the naive pluripotency network and specifically induces expression of Plakoglobin (Jup), a vertebrate homolog of β-catenin. Overexpression of Plakoglobin is sufficient to fully re-establish the naive pluripotency gene regulatory network under metastable pluripotency conditions, as confirmed by single-cell transcriptome profiling. Fi","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-07-07T03:09:40.708Z","creation":"2025-04-05T20:29:14.666Z"},"accession":"S-EPMC10329048","cross_references":{"pubmed":["37419903"],"doi":["10.1038/s41467-023-39515-0"]}}