biostudies-literatureZhang KLthe Science and Technology Foundation in Guangdong Provincethe Fundamental Research Funds for the Central Universitiesthe National Natural Science Foundation of Guangdong ProvinceNational Natural Science Foundation of Chinathe Shenzhen Fundamental Research Programthe Science and Technology Foundation in Guangzhou Citythe Futian District Health and Public Welfare Research Project of Shenzhen City2989-3003https://www.ebi.ac.uk/biostudies/studies/S-EPMC10333378biostudies-literatureUnknown43(6)Elabela (ELA), which is the second endogenous peptide ligand of the apelin receptor (APJ) to be discovered, has been widely studied for potential use as a therapeutic peptide. However, its role in ischemic stroke (IS), which is a leading cause of disability and death worldwide and has limited therapeutic options, is uncertain. The aim of the present study was to investigate the beneficial effects of ELA on neuron survival after ischemia and the underlying molecular mechanisms. Primary cortical neurons were isolated from the cerebral cortex of pregnant C57BL/6J mice. Flow cytometry and immunofluorescence showed that ELA inhibited oxygen-glucose deprivation (OGD) -induced apoptosis and axonal damage in vitro. Additionally, analysis of the Gene Expression Omnibus database revealed that the exCellular and molecular neurobiologyElabela, a Novel Peptide, Exerts Neuroprotective Effects Against Ischemic Stroke Through the APJ/miR-124-3p/CTDSP1/AKT Pathway.PMC10333378No. 2017A030313503No. FTWS2019001, FTWS2021016, FTWS2022018No. 81070125, 81270213, 81670306No. 13ykzd16, 17ykjc18No. JCYJ20190808101405466, JCYJ20210324115003008, JCYJ20220530144404009No. 201806020084No. 2010B031600032, 2014A020211002Zhang KLZhou CQFu JYWu HDLi SMHong YHWang TChen XXZheng GHZeng CTHou JYWu HfalseElabela, a Novel Peptide, Exerts Neuroprotective Effects Against Ischemic Stroke Through the APJ/miR-124-3p/CTDSP1/AKT Pathway.Elabela (ELA), which is the second endogenous peptide ligand of the apelin receptor (APJ) to be discovered, has been widely studied for potential use as a therapeutic peptide. However, its role in ischemic stroke (IS), which is a leading cause of disability and death worldwide and has limited therapeutic options, is uncertain. The aim of the present study was to investigate the beneficial effects of ELA on neuron survival after ischemia and the underlying molecular mechanisms. Primary cortical neurons were isolated from the cerebral cortex of pregnant C57BL/6J mice. Flow cytometry and immunofluorescence showed that ELA inhibited oxygen-glucose deprivation (OGD) -induced apoptosis and axonal damage in vitro. Additionally, analysis of the Gene Expression Omnibus database revealed that the ex2023-01-01T00:00:00Z2023 Aug2026-05-29T00:14:15.372Z2025-04-04T20:29:22.407ZS-EPMC103333783710627210.1007/s10571-023-01352-6